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BMC Cell Biology

, 15:8

Cytoplasmic and organelle biology

Abstract

BackgroundMyosin IC is a single headed member of the myosin superfamily that localizes to the cytoplasm and the nucleus and is implicated in a variety of processes in both compartments. We recently identified a novel isoform of myosin IC and showed that the MYOIC gene in mammalian cells encodes three isoforms isoforms A, B, and C that differ only in the addition of short isoform-specific N-terminal peptides. The expression pattern of the isoforms and the mechanisms of expression regulation remain unknown.

ResultsTo determine the expression patterns of myosin IC isoforms, we performed a comprehensive expression analysis of the two myosin IC isoforms isoform A and B that contain isoform-specific sequences. By immunoblotting with isoform-specific antibodies and by qRT-PCR with isoform-specific primer we demonstrate that myosin IC isoforms A and B have distinct expression patterns in mouse tissues. Specifically, we show that myosin IC isoform A is expressed in a tissue specific pattern, while myosin IC isoform B is ubiquitously expressed at comparable levels in mouse tissues.

ConclusionsThe differences in the expression profile of the myosin IC isoforms indicate a tissue-specific MYOIC gene regulation and further suggest that the myosin IC isoforms, despite their high sequence homology, might have tissue-specific and isoform-specific functions.

KeywordsMyosin IC Isoforms Gene expression Tissue specificity Protein expression AbbreviationsRT-PCRreverse transcription polymerase chain reaction

qRT-PCRquantitative real time polymerase chain reaction

SDS-PAGEsodium dodecyl sulfate-polyacrylamide gel electrophoresis

PBSphosphate buffered saline

5’ UTR5’ untranslated region.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-15-8 contains supplementary material, which is available to authorized users.

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Autor: Neil L Sielski - Ivanna Ihnatovych - Jacob J Hagen - Wilma A Hofmann

Fuente: https://link.springer.com/



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