Cigarette smoke condensate increases C. albicans adhesion, growth, biofilm formation, and EAP1, HWP1 and SAP2 gene expressionReportar como inadecuado




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BMC Microbiology

, 14:61

Ecological and evolutionary microbiology

Abstract

BackgroundSmokers are more prone to oral infections than are non-smokers. Cigarette smoke reaches the host cells but also microorganisms present in the oral cavity. The contact between cigarette smoke and oral bacteria promotes such oral diseases as periodontitis. Cigarette smoke can also modulate C. albicans activities that promote oral candidiasis. The goal of this study was to investigate the effect of cigarette smoke condensate on C. albicans adhesion, growth, and biofilm formation as well as the activation of EAP1, HWP1 and secreted aspartic protease 2.

ResultsCigarette smoke condensate CSC increased C. albicans adhesion and growth, as well as biofilm formation. These features may be supported by the activation of certain important genes. Using quantitative RT-PCR, we demonstrated that CSC-exposed C. albicans expressed high levels of EAP1, HWP1 and SAP2 mRNA and that this gene expression increased with increasing concentrations of CSC.

ConclusionCSC induction of C. albicans adhesion, growth, and biofilm formation may explain the increased persistence of this pathogen in smokers. These findings may also be relevant to other biofilm-induced oral diseases.

KeywordsCigarette smoke Tobacco C albicans Adhesion Growth Biofilm Genes EAP-1 HWP-1 Sap2 Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-14-61 contains supplementary material, which is available to authorized users.

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Autor: Abdelhabib Semlali - Kerstin Killer - Humidah Alanazi - Witold Chmielewski - Mahmoud Rouabhia

Fuente: https://link.springer.com/



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