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Journal of Applied Genetics

, Volume 55, Issue 2, pp 267–271

First Online: 25 February 2014Received: 08 July 2013Revised: 29 January 2014Accepted: 04 February 2014DOI: 10.1007-s13353-014-0199-8

Cite this article as: Kich, J.D., Uthe, J.J., Benavides, M.V. et al. J Appl Genetics 2014 55: 267. doi:10.1007-s13353-014-0199-8


Toll-like receptor 4 TLR4 is a key factor in the innate immune recognition of lipopolysaccharide LPS from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host’s immune response to bacterial infections. The current study screened for single nucleotide polymorphisms SNPs in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs n = 117 were intranasally challenged at 7 weeks of age with 1 × 10 CFU of S. Typhimurium χ4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20-21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low n = 20 and persistent n = 20 Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms SNPs in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5′ untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of Salmonella fecal shedding, and seven of those SNPs were also associated with a quantitative measurement of fecal shedding P < 0.05. Using a stepwise regression process, a haplotype composed of SNPs rs80787918 and rs80907449 P ≤ 4.0 × 10 spanning a region of 4.9 Kb was identified, thereby providing additional information of the influence of those SNPs on Salmonella fecal shedding in pigs.

KeywordsSalmonella Single nucleotide polymorphisms SNPs Swine TLR4 Electronic supplementary materialThe online version of this article doi:10.1007-s13353-014-0199-8 contains supplementary material, which is available to authorized users.

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Autor: Jalusa Deon Kich - Jolita Janutenaite Uthe - Magda Vieira Benavides - Maurício Egídio Cantão - Ricardo Zanella - Chris


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