Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial CellsReportar como inadecuado

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Journal of Diabetes Research - Volume 2016 2016, Article ID 3537863, 11 pages -

Research Article

Kidney Disease Program, Department of Medicine, University of Louisville, Louisville, KY 40202, USA

Harvard Medical School, Boston, MA 02115, USA

Tuskegee University School of Veterinary Medicine, Tuskegee, AL 36088, USA

Received 9 August 2015; Revised 22 November 2015; Accepted 30 November 2015

Academic Editor: Feng Zheng

Copyright © 2016 Michelle T. Barati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The effects of acute exposure to high glucose levels as experienced by glomerular mesangial cells in postprandial conditions and states such as in prediabetes were investigated using proteomic methods. Two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry methods were used to identify protein expression patterns in immortalized rat mesangial cells altered by 2 h high glucose HG growth conditions as compared to isoosmotic-normal glucose control conditions. Unique protein expression changes at 2 h HG treatment were measured for 51 protein spots. These proteins could be broadly grouped into two categories: 1 proteins involved in cell survival-cell signaling and 2 proteins involved in stress response. Immunoblot experiments for a protein belonging to both categories, prohibitin PHB, supported a trend for increased total expression as well as significant increases in an acidic PHB isoform. Additional studies confirmed the regulation of proteasomal subunit alpha-type 2 and the endoplasmic reticulum chaperone and oxidoreductase PDI protein disulfide isomerase, suggesting altered ER protein folding capacity and proteasomal function in response to acute HG. We conclude that short term high glucose induces subtle changes in protein abundances suggesting posttranslational modifications and regulation of pathways involved in proteostasis.

Autor: Michelle T. Barati, James C. Gould, Sarah A. Salyer, Susan Isaacs, Daniel W. Wilkey, and Michael L. Merchant



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