Antioxidant Activity and Characterization of One New Polysaccharide Obtained from Perigord Truffle Tuber huidongenseReportar como inadecuado




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Evidence-Based Complementary and Alternative Medicine - Volume 2016 2016, Article ID 3537193, 7 pages -

Research Article

Department of Basic Medical Sciences, School of Medicine, Taizhou University, Taizhou 318000, Zhejiang, China

Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, China

Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Jiangsu 210009, China

Department of Food Science, Southwest University, Chongqing 400715, China

Received 17 March 2016; Revised 19 May 2016; Accepted 7 June 2016

Academic Editor: Gabriel A. Agbor

Copyright © 2016 Yong-Feng Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

As a medicinal and edible fungus parasitizing on the trees, Perigord Truffle Tuber huidongense is well known for its delicious taste, unique smell, and high medical value for healthcare. One new water-soluble nonstarch polysaccharide PST-W with the yield of 0.41% from Perigord Truffle Tuber huidongense was purified and identified on structural characteristics for the first time. The characterizations of PST-W were studied on physicochemical properties, main components of monosaccharides, and molecular structure. The monosaccharide compositions of PST-W were studied and identified as glucan, only containing D-glucoses with the molecular structure of →6 α-D-Glcp   α-D-Glcp 1→n by methylation analysis and NMR. In the determination of total reducing capacity, the reducing abilities of polysaccharide extracts could be listed as vitamin C > PST-W > crude polysaccharides-3 > crude polysaccharides-2 > crude polysaccharides-1. All of PST-W, crude polysaccharides-2, and crude polysaccharides-3 were relatively good scavenger for 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-2,4,6-trinitrophenylhydrazyl radicals with IC50 of 2.81, 4.17, and 3.44 mg-mL, respectively. However, clearing abilities of PST-W and crude polysaccharides were obviously weaker. The activities of total crude extract were the worst, indicating that the impurities might negatively affect the antioxidant activity. Thus, the separation and purification of polysaccharides were significant to increase the antioxidant activity in some degree.





Autor: Yong-Feng Chen, Wen-wen Jiang, Shi-qi Zhang, Jian-quan Kan, and Yong Liang

Fuente: https://www.hindawi.com/



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