Evidence for methionine-sulfoxide-reductase gene transfer from Alphaproteobacteria to the transcriptionally active macronucleus of the ciliate, Euplotes raikoviReportar como inadecuado




Evidence for methionine-sulfoxide-reductase gene transfer from Alphaproteobacteria to the transcriptionally active macronucleus of the ciliate, Euplotes raikovi - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 14:288

Ecological and evolutionary microbiology

Abstract

BackgroundDeleterious phenomena of protein oxidation affect every aerobic organism and methionine residues are their elective targets. The reduction of methionine sulfoxides back to methionines is catalyzed by methionine-sulfoxide reductases Msrs, enzymes which are particularly active in microorganisms because of their unique nature of individual cells directly exposed to environmental oxidation.

ResultsFrom the transcriptionally active somatic genome of a common free-living marine protist ciliate, Euplotes raikovi, we cloned multiple gene isoforms encoding Msr of type A MsrA committed to repair methionine-S-sulfoxides. One of these isoforms, in addition to including a MsrA-specific nucleotide sequence, included also a sequence specific for a Msr of type B MsrB committed to repair methionine-R-sulfoxides. Analyzed for its structural relationships with MsrA and MsrB coding sequences of other organisms, the coding region of this gene named msrAB showed much more significant relationships with Msr gene coding sequences of Rhodobacterales and Rhizobiales Alphaproteobacteria, than of other eukaryotic organisms.

ConclusionsBased on the fact that the msrAB gene is delimited by Euplotes-specific regulatory 5’ and 3’ regions and telomeric C4A4-G4T4 repeats, it was concluded that E. raikovi inherited the coding region of this gene through a phenomenon of horizontal gene transfer from species of Alphaproteobacteria with which it coexists in nature and on which it likely feeds.

KeywordsHorizontal gene transfer Protozoa Alphaproteobacteria Gene structure Methionine sulfoxide reductases Electronic supplementary materialThe online version of this article doi:10.1186-s12866-014-0288-1 contains supplementary material, which is available to authorized users.

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Autor: Nicoleta Dobri - Annalisa Candelori - Francesca Ricci - Pierangelo Luporini - Adriana Vallesi

Fuente: https://link.springer.com/







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