The promoter of cell growth- and RNA protection-associated SND1 gene is activated by endoplasmic reticulum stress in human hepatoma cells Report as inadecuate

The promoter of cell growth- and RNA protection-associated SND1 gene is activated by endoplasmic reticulum stress in human hepatoma cells - Download this document for free, or read online. Document in PDF available to download.

BMC Biochemistry

, 15:25

Nucleic acids biochemistry


BackgroundStaphyloccocal nuclease domain-containing protein 1 SND1 is involved in the regulation of gene expression and RNA protection. While numerous studies have established that SND1 protein expression is modulated by cellular stresses associated with tumor growth, hypoxia, inflammation, heat-shock and oxidative conditions, little is known about the factors responsible for SND1 expression. Here, we have approached this question by analyzing the transcriptional response of human SND1 gene to pharmacological endoplasmic reticulum ER stress in liver cancer cells.

ResultsWe provide first evidence that SND1 promoter activity is increased in human liver cancer cells upon exposure to thapsigargin or tunicamycin or by ectopic expression of ATF6, a crucial transcription factor in the unfolded protein response triggered by ER stress. Deletion analysis of the 5’-flanking region of SND1 promoter identified maximal activation in fragment -934, +221, which contains most of the predicted ER stress response elements in proximal promoter. Quantitative real-time PCR revealed a near 3 fold increase in SND1 mRNA expression by either of the stress-inducers; whereas SND1 protein was maximally upregulated 3.4-fold in cells exposed to tunicamycin, a protein glycosylation inhibitor.

ConclusionPromoter activity of the cell growth- and RNA-protection associated SND1 gene is up-regulated by ER stress in human hepatoma cells.

KeywordsSND1 transcriptional activity Tudor and nuclease domain containing protein 1 Tudor-SN ER stress response ATF6 AbbreviationsATF6Activating transcription factor 6

CHOPC-EBP homologous protein

eIF2αEukaryotic initiation factor 2 alpha

EREndoplasmic reticulum

ERSEER stress response element

GRP78Glucose-regulated protein 78

GRP94Glucose-regulated protein 94

IRE1Inositol-requiring transmembrane kinase-endonuclease protein-1


PERKProtein kinase RNA-like ER kinase

SND1Staphylococcal nuclease and Tudor domain-containing protein 1

UPRUnfolded protein response

XBP1X-box binding protein 1

Electronic supplementary materialThe online version of this article doi:10.1186-s12858-014-0025-2 contains supplementary material, which is available to authorized users.

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Author: Sandra Armengol - Enara Arretxe - Leire Enzunza - Sarai Mula - Begoña Ochoa - Yolanda Chico - María José Martínez



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