Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirsReportar como inadecuado




Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 14:315

Clinical microbiology and vaccines

Abstract

BackgroundPreliminary studies showed the prevalence of a virus similar to human hepatitis B virus HBV-like in swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil. In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential.

ResultsBile and liver samples from swine were positive for partial genome amplification ORF S and ORF C, direct sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the presence of HBsAg antigen in liver tissue sections.

ConclusionsSo far our data suggest that commercial swine house an HBV-like virus and this relevant finding should be considered in studies on the origin and viral evolution.

KeywordsHepadnavirus Brazil Commercial swine Bile Liver Genotyping Immunofluorescence AbbreviationsHBVHuman hepatitis B virus

ORF SOpen reading frame S

ORF COpen reading frame C

WHVHBV-related virus of woodchuck

GSHVHBV-related virus of ground squirrels

ASHVHBV-related virus of arctic squirrels

DHBVHBV-related virus of ducks

GHBVHBV-related virus of geese

HHBVHBV-related virus of herons

STHBVHBV-related virus of storks

HBsAgSurface antigen of HBV

anti-HBsHBV surface antibody

anti-HBcAntibody to HBV core antigen

bpBase pair

CEUACommittee for Ethics in the Use of Research Animals

ASPAAnimal Sanitary Protection Agency in Rio de Janeiro State

ntNucleotide

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-014-0315-2 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Yasmine Rangel Vieira - Debora Regina Lopes dos Santos - Moyra Machado Portilho - Carlos Eduardo Pereira Velloso - Marci

Fuente: https://link.springer.com/







Documentos relacionados