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BMC Genetics

, 15:155

Complex traits and quantitative genetics


BackgroundSoil phosphorus P deficiency is one of the major limiting factors to crop production. The development of crop varieties with improved P use efficiency PUE is an important strategy for sustainable agriculture. The objectives of this research were to identify quantitative trait loci QTLs linked to PUE traits using a high-density single nucleotide polymorphism SNP map and to estimate the epistatic interactions and environmental effects in rice Oryza sativa L

ResultsWe conducted a two-year field experiment under low and normal P conditions using a recombinant inbred population of rice derived from Zhenshan 97 and Minghui 63 indica. We investigated three yield traits, biomass BIOM, harvest index HI, and grain yield Yield, and eight PUE traits: total P uptake PUP, P harvest index PHI, grain P use efficiency gPUE based on P accumulation in grains, straw P use efficiency strPUE based on P accumulation in straw, P use efficiency for biomass PUEb and for grain yield PUEg based on P accumulation in the whole plant, P translocation PT, and P translocation efficiency PTE. Of the 36 QTLs and 24 epistatic interactions identified, 26 QTLs and 12 interactions were detected for PUE traits. The environment affected seven QTLs and three epistatic interactions. Four QTLs qPHI1 and qPHI2 for PHI, qPUEg2 for PUEg, and qPTE8 for PTE with strong effects were environmentally independent. By comparing our results with similar QTLs in previous studies, three QTLs for PUE traits qPUP1 and qPUP10 for PUP, and qPHI6 for PHI were found across various genetic backgrounds. Seven regions were shared by QTLs for yield and PUE traits.

ConclusionMost QTLs linked to PUE traits were different from those linked to yield traits, suggesting different genetic controls underlying these two traits. Those chromosomal regions with large effects that are not affected by different environments are promising for improving P use efficiency. The seven regions shared by QTLs linked to yield and PUE traits imply the possibility of the simultaneous improvement of yield and PUE traits.

KeywordsGenotype by environment interaction Phosphorus use efficiency Quantitative trait loci Recombinant inbred lines Rice Single nucleotide polymorphism AbbreviationsBIOMBiomass

gPUEGrain P use efficiency based on P accumulation in grains

HIHarvest index

PHIP harvest index

PTP translocation

PTEP translocation efficiency

PUEbP use efficiency for biomass

PUEgP use efficiency for grain yield based on P accumulation in the whole plant

PUPTotal P uptake in the whole plant

QTLsQuantitative trait loci

strPUEStraw P use efficiency based on P accumulation in straw

Electronic supplementary materialThe online version of this article doi:10.1186-s12863-014-0155-y contains supplementary material, which is available to authorized users.

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Autor: Kai Wang - Kehui Cui - Guoling Liu - Weibo Xie - Huihui Yu - Junfeng Pan - Jianliang Huang - Lixiao Nie - Farooq Shah - S


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