CLIP: viewing the RNA world from an RNA-protein interactome perspectiveReport as inadecuate

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Science China Life Sciences

, Volume 58, Issue 1, pp 75–88

First Online: 10 January 2015Received: 14 July 2014Accepted: 13 August 2014DOI: 10.1007-s11427-014-4764-5

Cite this article as: Zhang, Y., Xie, S., Xu, H. et al. Sci. China Life Sci. 2015 58: 75. doi:10.1007-s11427-014-4764-5


The pervasive transcription of the genome creates many types of non-coding RNAs ncRNAs. However, we know very little regarding the functions and the regulatory mechanisms of these ncRNAs. Exploring the interactions of RNA and RNA binding proteins RBPs is vital because it can allow us to truly understand how these ncRNAs behave in vivo. High-throughput sequencing of RNA isolated by cross-linking immunoprecipitation HITS-CLIP or CLIP-seq and its variants have been successfully used as systemic techniques to study RBP binding sites. In this review, we will explain the major differences between the CLIP techniques, summarize successful applications of these techniques, discuss limitations of CLIP, present some suggested solutions and project their promising future roles in studying the RNA world.

KeywordsCLIP CLASH RPBs ncRNAs functional RNomics This article is published with open access at

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Author: Yin Zhang - ShuJuan Xie - Hui Xu - LiangHu Qu


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