Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlingsReport as inadecuate

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BMC Microbiology

, 15:21

First Online: 06 February 2015Received: 31 March 2014Accepted: 19 January 2015DOI: 10.1186-s12866-015-0353-4

Cite this article as: Xie, S., Wu, H., Chen, L. et al. BMC Microbiol 2015 15: 21. doi:10.1186-s12866-015-0353-4


BackgroundPlant growth-promoting rhizobacteria PGPR are soil beneficial microorganisms that colonize plant roots for nutritional purposes and accordingly benefit plants by increasing plant growth or reducing disease. However, the mechanisms and pathways involved in the interactions between PGPR and plants remain unclear. In order to better understand these complex plant-PGPR interactions, changes in the transcriptome of the typical PGPR Bacillus subtilis in response to rice seedlings were analyzed.

ResultsMicroarray technology was used to study the global transcriptionl response of B. subtilis OKB105 to rice seedlings after an interaction period of 2 h. A total of 176 genes representing 3.8% of the B. subtilis strain OKB105 transcriptome showed significantly altered expression levels in response to rice seedlings. Among these, 52 were upregulated, the majority of which are involved in metabolism and transport of nutrients, and stress responses, including araA, ywkA, yfls, mtlA, ydgG et al. The 124 genes that were downregulated included cheV, fliL, spmA and tua, and these are involved in chemotaxis, motility, sporulation and teichuronic acid biosynthesis, respectively.

ConclusionsWe present a transcriptome analysis of the bacteria Bacillus subtilis OKB105 in response to rice seedings. Many of the 176 differentially expressed genes are likely to be involved in the interaction between Gram-positive bacteria and plants.

KeywordsBacillus subtilis Oryza sativa Plant-microbe interactions Transcriptomics Microarray Functional annotation Shanshan Xie, Huijun Wu contributed equally to this work.

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Author: Shanshan Xie - Huijun Wu - Lina Chen - Haoyu Zang - Yongli Xie - Xuewen Gao


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