Loss of heterozygosity for chromosomal regions 15q14-21.1, 17q21.31, and 13q12.3-13.1 and its relevance for prostate cancerReportar como inadecuado

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Medical Oncology

, 32:246

First Online: 03 October 2015Received: 23 September 2015Accepted: 24 September 2015DOI: 10.1007-s12032-015-0691-y

Cite this article as: Nowacka-Zawisza, M., Forma, E., Walczak, M. et al. Med Oncol 2015 32: 246. doi:10.1007-s12032-015-0691-y


Although prostate cancer is one of the most common cancers in men, the genetic defects underlying its pathogenesis remain poorly understood. DNA damage repair mechanisms have been implicated in human cancer. Accumulating evidence indicates that the fidelity of the response to DNA double-strand breaks is critical for maintaining genome integrity. RAD51 is a central player in double-strand break repair via homologous recombination, and its alterations may confer and increase the risk of cancer. RAD51 functioning depends on the indirect or direct interactions with BRCA1 and BRCA2. To evaluate the contribution of RAD51 to sporadic prostate cancer, loss of heterozygosity LOH for chromosomal region 15q14-21.1 RAD51locus was determined and compared to LOH in 17q21.31 BRCA1 locus and 13q12.3-13.1 BRCA2 region. DNA was isolated from prostate biopsies and matched peripheral blood of 50 patients. The regions 15q14-21.1, 17q21.31, and 13q12.3-13.1 were examined using microsatellite markers on chromosome 15 D15S118, D15S214, D15S1006, chromosome 17 D17S855, D17S1323, and chromosome 13 D13S260, D13S290, respectively. The LOH in tumors was analyzed by PCR with fluorescently labeled primers and an ABI PRISM 377 DNA Sequencer. Allele sizing was determined by GeneScan version 3.1.2 and Genotyper version 2.5 software Applied Biosystems, USA. LOH was identified in 57.5, 23, and 40 % for chromosomal regions 15q14-21.1, 17q21.31, and 13q12.3-13.1, respectively. Twenty-six percent of studied cases manifested LOH for at least one marker in 15q14-21.1 exclusively. A significant correlation was found between LOH for studied region and PSAD prostate-specific antigen density. The findings suggest that RAD51 may be considered as a prostate cancer susceptibility gene.

KeywordsLoss of heterozygosity LOH Prostate cancer RAD51 Molecular markers  Download fulltext PDF

Autor: Maria Nowacka-Zawisza - Ewa Forma - Maciej Walczak - Waldemar Różański - Magdalena Bryś - Wanda M. Krajewska

Fuente: https://link.springer.com/

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