Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreumReport as inadecuate

Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum - Download this document for free, or read online. Document in PDF available to download.

BMC Biotechnology

, 15:2

First Online: 07 February 2015Received: 23 September 2014Accepted: 23 January 2015DOI: 10.1186-s12896-015-0116-1

Cite this article as: Peña-Cardeña, A., Rodríguez-Alegría, M.E., Olvera, C. et al. BMC Biotechnol 2015 15: 2. doi:10.1186-s12896-015-0116-1


BackgroundIslA4 is a truncated single domain protein derived from the inulosucrase IslA, which is a multidomain fructosyltransferase produced by Leuconostoc citreum. IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity. IslA4 has been reported to retain the product specificity of the multidomain enzyme.

ResultsScreening experiments to evaluate the influence of the reactions conditions, especially the sucrose and enzyme concentrations, on IslA4 product specificity revealed that high sucrose concentrations shifted the specificity of the reaction towards fructooligosaccharides FOS synthesis, which almost eliminated inulin synthesis and led to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allowed for high levels of FOS synthesis, where 70% sucrose was used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS.

ConclusionsDomain truncation together with the selection of the appropriate reaction conditions resulted in the synthesis of various FOS, which were produced as the main transferase products of inulosucrase IslA4. These results therefore demonstrate that bacterial fructosyltransferase could be used for the synthesis of inulin-type FOS.

KeywordsFructosyltransferase Fructooligosaccharides Inulin Inulosucrase Leuconostoc citreum AbbreviationsDPDegree of polymerization



GPCGel permeation chromatography

HPAEC-PADHigh performance anion exchange chromatography with pulsed amperometric detection

TLCThin-layer chromatography

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Author: Arlen Peña-Cardeña - María Elena Rodríguez-Alegría - Clarita Olvera - Agustín López Munguía


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