Global analyses of Chromosome 17 and 18 genes of lung telocytes compared with mesenchymal stem cells, fibroblasts, alveolar type II cells, airway epithelial cells, and lymphocytesReportar como inadecuado

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Biology Direct

, 10:9

First Online: 11 March 2015Received: 09 October 2014Accepted: 10 February 2015DOI: 10.1186-s13062-015-0042-0

Cite this article as: Wang, J., Ye, L., Jin, M. et al. Biol Direct 2015 10: 9. doi:10.1186-s13062-015-0042-0


BackgroundTelocytes TCs is an interstitial cell with extremely long and thin telopodes Tps with thin segments podomers and dilations podoms to interact with neighboring cells. TCs have been found in different organs, while there is still a lack of TCs-specific biomarkers to distinguish TCs from the other cells.

ResultsWe compared gene expression profiles of murine pulmonary TCs on days 5 TC5 and days 10 TC10 with mesenchymal stem cells MSCs, fibroblasts Fbs, alveolar type II cells ATII, airway basal cells ABCs, proximal airway cells PACs, CD8 T cells from bronchial lymph nodes T-BL, and CD8 T cells from lungs T-LL. The chromosome 17 and 18 genes were extracted for further analysis. The TCs-specific genes and functional networks were identified and analyzed by bioinformatics tools. 16 and 10 of TCs-specific genes were up-regulated and 68 and 22 were down-regulated in chromosome 17 and 18, as compared with other cells respectively. Of them, Mapk14 and Trem2 were up-regulated to indicate the biological function of TCs in immune regulation, and up-regulated MCFD2 and down-regulated E4F1 and PDCD2 had an association with tissue homeostasis for TCs. Over-expressed Dpysl3 may promote TCs self-proliferation and cell-cell network forming.

ConclusionsThe differential gene expression in chromosomes 17 and 18 clearly revealed that TCs were the distinctive type of interstitial cells. Our data also indicates that TCs may play a dual role in immune surveillance and immune homoeostasis to keep from immune disorder in acute and chronic pulmonary diseases. TCs also participated in proliferation, differentiation and regeneration.

ReviewersThis article was reviewed by Qing Kay Li and Dragos Cretoiu.

KeywordsChromosome 17 Chromosome 18 Genes Lung Telocytes Mesenchymal stem cells Fibroblasts Alveolar type II cells Airwayepithelial cells Lymphocytes AbbreviationABCsAirway basal cells

ATIIAlveolar type II cells

DPYSL3Dihydropyrimidinase-like 3

E4F1E4F transcription factor 1


GEOGene Expression Omnibus

MAPK14Mitogen-activated protein kinase 14

MCFD2Multiple coagulation factor deficiency 2

MSCsMesenchymal stem cells

NCBINational Center for Biotechnology Information

PACsProximal airway cells

PDCD2Programmed cell death 2

T-BLCD8+ T cells from bronchial lymph nodes


TC5TCs on days 5

TC10TCs on days 10

TEMTransmission electron microscopy

T-LLCD8+ T cells from lungs


TREM2Triggering receptor expressed on myeloid cells 2

Jian Wang and Ling Ye contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s13062-015-0042-0 contains supplementary material, which is available to authorized users.

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Autor: Jian Wang - Ling Ye - Meiling Jin - Xiangdong Wang


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