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BMC Evolutionary Biology

, 15:41

First Online: 13 March 2015Received: 21 November 2014Accepted: 24 February 2015DOI: 10.1186-s12862-015-0316-2

Cite this article as: Svetec, N., Zhao, L., Saelao, P. et al. BMC Evol Biol 2015 15: 41. doi:10.1186-s12862-015-0316-2

Abstract

BackgroundDrosophila melanogaster often shows correlations between latitude and phenotypic or genetic variation on different continents, which suggests local adaptation with respect to a heterogeneous environment. Previous phenotypic analyses of latitudinal clines have investigated mainly physiological, morphological, or life-history traits. Here, we studied latitudinal variation in sleep in D. melanogaster populations from North and Central America. In parallel, we used RNA-seq to identify interpopulation gene expression differences.

ResultsWe found that in D. melanogaster the average nighttime sleep bout duration exhibits a latitudinal cline such that sleep bouts of equatorial populations are roughly twice as long as those of temperate populations. Interestingly, this pattern of latitudinal variation is not observed for any daytime measure of activity or sleep. We also found evidence for geographic variation for sunrise anticipation. Our RNA-seq experiment carried out on heads from a low and high latitude population identified a large number of gene expression differences, most of which were time dependent. Differentially expressed genes were enriched in circadian regulated genes and enriched in genes potentially under spatially varying selection.

ConclusionOur results are consistent with a mechanistic and selective decoupling of nighttime and daytime activity. Furthermore, the present study suggests that natural selection plays a major role in generating transcriptomic variation associated with circadian behaviors. Finally, we identified genomic variants plausibly causally associated with the observed behavioral and transcriptomic variation.

KeywordsDrosophila melanogaster Latitudinal cline Spatially varying selection Locomotor activity Sleep Circadian rhythms Gene expression RNA-seq AbbreviationsMEMaine

RIRhode Island

VAVirginia

FLFlorida

PCPanama City

FSTFixation index

GOGene ontology

LDLight-Dark

DDDark-Dark

ZTZeitgeber

BSEBehavioral State dependent expression

Electronic supplementary materialThe online version of this article doi:10.1186-s12862-015-0316-2 contains supplementary material, which is available to authorized users.

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Autor: Nicolas Svetec - Li Zhao - Perot Saelao - Joanna C Chiu - David J Begun

Fuente: https://link.springer.com/







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