Dexamethasone in osteogenic medium strongly induces adipocyte differentiation of mouse bone marrow stromal cells and increases osteoblast differentiationReportar como inadecuado




Dexamethasone in osteogenic medium strongly induces adipocyte differentiation of mouse bone marrow stromal cells and increases osteoblast differentiation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cell Biology

, 16:9

First Online: 13 March 2015Received: 17 November 2014Accepted: 19 February 2015DOI: 10.1186-s12860-015-0056-6

Cite this article as: Ghali, O., Broux, O., Falgayrac, G. et al. BMC Cell Biol 2015 16: 9. doi:10.1186-s12860-015-0056-6

Abstract

BackgroundOsteoblasts and adipocytes share a common mesenchymal stem cell origin. Therefore, it has been suggested that the accumulation of marrow adipocytes observed in bone loss is caused by a shift in the commitment of mesenchymal stem cells from the osteogenic pathway to the adipogenic pathway. Supporting this hypothesis the competition between adipogenic and osteogenic lineages was widely demonstrated on partially homogeneous cell populations. However, some data from mouse models showed the existence of an independent relationship between bone mineral content and bone marrow adiposity. Therefore, the combination of adipogenesis and osteogenesis in primary culture would be helpful to determine if this competition would be observed on a whole bone marrow stromal cell population in a culture medium allowing both lineages.

In this aim, mouse bone marrow stromal cells were cultured in a standard osteogenic medium added with different concentrations of Dexamethasone, known to be an important regulator of mesenchymal progenitor cell differentiation.

ResultsGene expression of osteoblast and adipocyte markers, biochemical and physical analyses demonstrated the presence of both cell types when Dexamethasone was used at 100 nM. Overall, our data showed that in this co-differentiation medium both differentiation lineages were enhanced compared to classical adipogenic or osteogenic culture medium. This suggests that in this model, adipocyte phenotype does not seem to increase at the expense of the osteoblast lineage.

ConclusionThis model appears to be a promising tool to study osteoblast and adipocyte differentiation capabilities and the interactions between these two processes.

KeywordsBone marrow stromal cells Cell differentiation Dexamethasone Co-differentiation medium Osteoblast Adipocyte Mouse AbbreviationsMSCMesenchymal stem cells

BMSCsBone marrow stromal cells

DexDexamethasone

Glut4Glucose transporter type 4

AdipoQAdiponectin

O100Osteogenic medium added with 100 nM of dexamethasone

Q-RT PCRQuantitative reverse transcription polymerase chain reaction

OCOsteocalcin

OSXOsterix, ALP, Alkaline phosphatase

α MEMAlpha minimum essential medium

FCSFoetal calf serum

DMEMDulbecco’s modified eagle medium

DAPI4′-6-Diamidino-2-phenylindole

PBSPhosphate buffered saline

HClHydrochloride acid

Ca2Calcium

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Autor: Olfa Ghali - Odile Broux - Guillaume Falgayrac - Nathalie Haren - Johannes PTM van Leeuwen - Guillaume Penel - Pierre Hard

Fuente: https://link.springer.com/







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