Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complexReportar como inadecuado

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BMC Genomics

, 16:376

First Online: 12 May 2015Received: 11 December 2014Accepted: 23 April 2015DOI: 10.1186-s12864-015-1564-7

Cite this article as: Soares, M.A., Araújo, R.A

, Marini, M.M. et al. BMC Genomics 2015 16: 376. doi:10.1186-s12864-015-1564-7


BackgroundSpecies from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes.

ResultsWe identified five different retrotransposon families four LTR-like and one LINE-like element in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii Pb01 but could not be detected in P. brasiliensis Pb03 and Pb18 by semi-quantitative RT-PCR.

ConclusionFive new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.

Marco Aurélio Soares and Roberta Amália de Carvalho Araújo contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-1564-7 contains supplementary material, which is available to authorized users.

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Autor: Marco Aurélio Soares - Roberta Amália de Carvalho Araújo - Marjorie Mendes Marini - Luciana Márcia de Oliveira - Leon


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