Antimicrobial activity and safety evaluation of Enterococcus faecium KQ 2.6 isolated from peacock fecesReportar como inadecuado




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BMC Biotechnology

, 15:30

First Online: 12 May 2015Received: 01 January 2015Accepted: 22 April 2015DOI: 10.1186-s12896-015-0151-y

Cite this article as: Zheng, W., Zhang, Y., Lu, HM. et al. BMC Biotechnol 2015 15: 30. doi:10.1186-s12896-015-0151-y

Abstract

BackgroundThe objective of this paper was to study antimicrobial activity and safety of Enterococcus faecium KQ 2.6 E. faecium KQ 2.6 isolated from peacock feces.

MethodsAgar well diffusion method was adopted in antimicrobial activity assay. Disk diffusion test was used to determine the antibiotic resistance. The identification and virulence potential of E. faecium KQ 2.6 were investigated using PCR amplification.

ResultsThe results indicated that cell free supernatant CFS of the strain had the good antimicrobial activity against selected gram-positive and gram-negative bacteria. The biochemical characteristics of antimicrobial substances were investigated. The results indicated that the antimicrobial substances were still active after treatment with catalase and proteinase, respectively. Moreover, the stability of antimicrobial substances did not change after heat treatment at 40, 50, 60, 70 and 80°C for 30 min, respectively. The activity of antimicrobial substances remained stable at 4 and −20°C after long time storage. The antimicrobial activity of CFS was compared with that of the buffer with similar strength and pH. The inhibitory zone of the buffer was apparently smaller than that of CFS, which meant that the acid in CFS was not the only factor that was contributed to antibacterial activity of CFS. The antibiotic resistance and virulence potential were evaluated using disk diffusion test and PCR amplification. The results showed that E. faecium KQ 2.6 did not harbor any tested virulence genes such as gelE, esp, asa1, cylA, efaA and hyl. It was susceptible to most of tested antibiotics except for vancomycin and polymyxin B.

ConclusionE. faecium KQ 2.6 may be used as bio-preservative cultures for the production of fermented foods.

KeywordsE. faecium KQ 2.6 Antimicrobial activity Safety evaluation Antibiotics resistance Virulence genes AbbreviationE. faecium KQ 2.6Enterococcus faecium KQ 2.6

E. faecalisEnterococci faecalis

CFSCell free supernatant

BLISBacteriocin like inhibitory substances

LABLactic acid bacteria

VREVancomycin-resistant enterococci

LBLuria-Bertani broth

MRSde Man Rogosa Sharpe agar

PDAPotato Dextrose Agar

CLSIClinical and Laboratory Standards Institute

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Autor: Wei Zheng - Yu Zhang - Hui-Min Lu - Dan-Ting Li - Zhi-Liang Zhang - Zhen-Xing Tang - Lu-E Shi

Fuente: https://link.springer.com/







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