Morphologically defined sub-stages of C. elegans vulval development in the fourth larval stageReportar como inadecuado

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BMC Developmental Biology

, 15:26

First Online: 12 June 2015Received: 18 February 2015Accepted: 03 June 2015DOI: 10.1186-s12861-015-0076-7

Cite this article as: Mok, D.Z., Sternberg, P.W. & Inoue, T. BMC Dev Biol 2015 15: 26. doi:10.1186-s12861-015-0076-7


BackgroundDuring the fourth larval L4 stage, vulval cells of C. elegans undergo extensive morphogenesis accompanied by changes in gene expression. This phase of vulval development, occurring after the well-studied induction of vulval cells, is not well understood but is potentially a useful context in which to study how a complex temporal sequence of events is regulated during development. However, a system for precisely describing different phases of vulval development in the L4 stage has been lacking.

ResultsWe defined ten sub-stages of L4 based on morphological criteria as observed using Nomarski microscopy L4.0 ~ L4.9. Precise timing of each sub-stage at 20 °C was determined. We also re-examined the timing of expression for several gene expression markers, and correlated the sub-stages with the timing of other developmental events in the vulva and the uterus.

ConclusionsThis scheme allows the developmental timing of an L4 individual to be determined at approximately one-hour resolution without the need to resort to time course experiments. These well-defined developmental stages will enable more precise description of gene expression and other developmental events.

KeywordsC. elegans Vulval development Developmental timing Gene expression  Download fulltext PDF

Autor: Darren Z. L. Mok - Paul W. Sternberg - Takao Inoue


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