In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via14C-TdR labelingReportar como inadecuado




In vivo tracking of human placenta derived mesenchymal stem cells in nude mice via14C-TdR labeling - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Biotechnology

, 15:55

First Online: 13 June 2015Received: 08 October 2014Accepted: 29 May 2015DOI: 10.1186-s12896-015-0174-4

Cite this article as: Wu, CG., Zhang, JC., Xie, CQ. et al. BMC Biotechnol 2015 15: 55. doi:10.1186-s12896-015-0174-4

Abstract

BackgroundIn order to shed light on the regenerative mechanism of mesenchymal stem cells MSCs in vivo, the bio-distribution profile of implanted cells using a stable and long-term tracking method is needed. We herein investigated the bio-distribution of human placental deciduas basalis derived MSCs termed as PDB-MSCs in nude mice after intravenous injection by carbon radioisotope labeling thymidine C-TdR, which is able to incorporate into new DNA strands during cell replication.

ResultsThe proliferation rate and radioactive emission of human PDB-MSCs after labeled with different concentrations of C-TdR were measured. PDB-MSCs labeled with 1 μCi possessed high radioactivity, and the biological characteristics i.e. morphology, colony forming ability, differentiation capabilities, karyotype and cell cycle showed no significant changes after labeling. Thus, 1 μCi was the optimal concentration in this experimental design. In nude mice, 1 × 10C-TdR-labeled PDB-MSCs were injected intravenously and the organs were collected at days 1, 2, 3, 5, 30 and 180 after injection, respectively. Radiolabeled PDB-MSCs were found mainly in the lung, liver, spleen, stomach and left femur of the recipient nude mice at the whole observation period.

ConclusionsThis work provided solid evidence that C-TdR labeling did not alter the biological characteristics of human placental MSCs, and that this labeling method has potential to decrease the signal from non-infused or dead cells for cell tracking. Therefore, this labeling technique can be utilized to quantify the infused cells after long-term follow-up in pre-clinical studies.

AbbreviationsMSCsMesenchymal stem cells

PDB-MSCsHuman placental deciduas basalis derived MSCs

C-TdRCarbon radioisotope labeling thymidine

MRIMagnetic resonance imaging scans

QDsQuantum dots

H-TdRTritiated thymidine

dpmDisintegrations per minute

CFUColony forming units

SDStandard deviation

PETPositron emission tomography

SPECTSingle photon emission computed tomography

RT-PCRReal time PCR

DMEM-HGDulbecco’s modified Eagle’s medium-High Glucose

FBSFetal bovine serum

PBSPhosphate buffer saline

EDTAEthylene diamine tetraacetic acid

DAB3,3′-diaminobenzidine

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Autor: Cheng-Guang Wu - Ji-Chun Zhang - Cheng-Quan Xie - Ornella Parolini - Antonietta Silini - Yi-Zhou Huang - Bing Lian - Min Z

Fuente: https://link.springer.com/







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