Sex and parasites: genomic and transcriptomic analysis of Microbotryum lychnidis-dioicae, the biotrophic and plant-castrating anther smut fungusReportar como inadecuado




Sex and parasites: genomic and transcriptomic analysis of Microbotryum lychnidis-dioicae, the biotrophic and plant-castrating anther smut fungus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genomics

, 16:461

First Online: 16 June 2015Received: 18 December 2014Accepted: 28 May 2015DOI: 10.1186-s12864-015-1660-8

Cite this article as: Perlin, M.H., Amselem, J., Fontanillas, E. et al. BMC Genomics 2015 16: 461. doi:10.1186-s12864-015-1660-8

Abstract

BackgroundThe genus Microbotryum includes plant pathogenic fungi afflicting a wide variety of hosts with anther smut disease. Microbotryum lychnidis-dioicae infects Silene latifolia and replaces host pollen with fungal spores, exhibiting biotrophy and necrosis associated with altering plant development.

ResultsWe determined the haploid genome sequence for M. lychnidis-dioicae and analyzed whole transcriptome data from plant infections and other stages of the fungal lifecycle, revealing the inventory and expression level of genes that facilitate pathogenic growth. Compared to related fungi, an expanded number of major facilitator superfamily transporters and secretory lipases were detected; lipase gene expression was found to be altered by exposure to lipid compounds, which signaled a switch to dikaryotic, pathogenic growth. In addition, while enzymes to digest cellulose, xylan, xyloglucan, and highly substituted forms of pectin were absent, along with depletion of peroxidases and superoxide dismutases that protect the fungus from oxidative stress, the repertoire of glycosyltransferases and of enzymes that could manipulate host development has expanded. A total of 14 % of the genome was categorized as repetitive sequences. Transposable elements have accumulated in mating-type chromosomal regions and were also associated across the genome with gene clusters of small secreted proteins, which may mediate host interactions.

ConclusionsThe unique absence of enzyme classes for plant cell wall degradation and maintenance of enzymes that break down components of pollen tubes and flowers provides a striking example of biotrophic host adaptation.

KeywordsMicrobotryum violaceum Anther smuts CAZymes Transposable elements Mating-type chromosomes Pathogen alteration of host development Joelle Amselem, Eric Fontanillas, Su San Toh, Zehua Chen and Michael E. Hood contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-1660-8 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Michael H Perlin - Joelle Amselem - Eric Fontanillas - Su San Toh - Zehua Chen - Jonathan Goldberg - Sebastien Duplessis

Fuente: https://link.springer.com/







Documentos relacionados