A new allele of acid soil tolerance gene from a malting barley varietyReportar como inadecuado

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BMC Genetics

, 16:92

Functional genetics


BackgroundAcid soil is a serious limitation to crop production all over the world. Toxic aluminium Al cations in acid soil inhibit root growth and reduce yield. Although a gene tolerant to acid soil has been identified, it has not been used in malting barley breeding, which is partly due to the acid soil tolerance gene being linked to unfavorable malting quality traits.

ResultsA Brazilian malting barley variety Br2 was identified as tolerant to acid soil. A doubled haploid DH population was developed from a cross between Br2 and the Australian acid-sensitive cultivar Hamelin. The DH population was tested for acid soil tolerance in native acid soil and a hydroponic system with pH 4.2, pH 4.2 + Al or pH 6.5, and genotyped using SSR, DArT and gene-specific markers. A single QTL was detected for all parameters related to acid soil tolerance. The QTL was mapped to the known HvMATE location on chromosome 4H. Sequence alignment of the HvMATE gene identified 13 INDELs and 87 SNPs, where one SNP coded for a single amino acid difference between the two varieties. A gene-specific marker was developed to detect the single nucleotide polymorphism between Hamelin and Br2. This marker co-segregated with aluminium tolerance and accounted for 79 % of phenotypic variation for acid soil tolerance.

ConclusionThe present study identified a novel source of acid soil-Al tolerance from a Brazilian malting barley cultivar Br2. This variety tolerated Al toxicity but was sensitive to low pH which is similar to most other Al-tolerant varieties. A gene-specific marker Cit7 was developed based on the HvMATE gene sequence. Cit7 will improve the efficiency of molecular-assisted selection of new barley varieties with tolerance to acid soil. Multiple alleles exist for the acid soil tolerance gene on chromosome 4H, so a malting barley variety that tolerates acid soil could be developed by selecting suitable tolerant alleles. Tolerance to low pH may play an important role for barley to adapt to acid soils.

KeywordsMalting barley Citrate transporter Aluminium tolerance Gene-specific marker AbbreviationsAlAluminium

ALMT1Aluminium-activated malate transporter 1

AtBCBArabidopsis blue copper binding protein

parBTobacco glutathione S-transferase

NtPOXTobacco peroxidase

NtGDI1Tobacco GDP-dissociation inhibitor

clf-59Curly leaf

FLCFlowering locus C



SNPSingle nucleotide polymorphism

HvAACTHordeum vulgare aluminium-activated citrate transporter

HvMATEHordeum vulgare multidrug and toxin extrusion

TaALMTWheat aluminium-activated malate transporter

ScAACT1Secale cereal aluminium-activated citrate transporter

AtALMT1Arabidopsis aluminium-activated malate transporter

PCRPolymerase chain reaction


DArTDiversity arrays technology

SSCPSingle-strand conformation polymorphism

SNPSingle nucleotide polymorphism

bpBase pair

AltAluminium tolerance

PhtTolerance to low pH

AlpTolerance to aluminium and low pH

Alp3Tolerance to aluminium and low pH 3

UTRUntranslated region

Miao Bian and Xiaoli Jin contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12863-015-0254-4 contains supplementary material, which is available to authorized users.

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Autor: Miao Bian - Xiaoli Jin - Sue Broughton - Xiao-Qi Zhang - Gaofeng  Zhou - Meixue Zhou - Guoping Zhang - Dongfa Sun - Chengd

Fuente: https://link.springer.com/

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