Flow cytometry remission by Ig light chains ratio is a powerful marker of outcome in multiple myeloma after tandem autologous transplant: a real-life studyReportar como inadecuado




Flow cytometry remission by Ig light chains ratio is a powerful marker of outcome in multiple myeloma after tandem autologous transplant: a real-life study - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Experimental and Clinical Cancer Research

, 35:49

First Online: 19 March 2016Received: 04 February 2016Accepted: 11 March 2016DOI: 10.1186-s13046-016-0324-0

Cite this article as: Cordone, I., Marchesi, F., Masi, S. et al. J Exp Clin Cancer Res 2016 35: 49. doi:10.1186-s13046-016-0324-0

Abstract

BackgroundThe achievement of complete response CR significantly correlates with a better clinical outcome in multiple myeloma MM patients treated with autologous stem cell transplant ASCT. The depth of response is one of the most relevant factors to predict patient’s outcome, however the definition of CR through standard criteria has shown several limitations.

MethodsIn this study we evaluated the minimal residual disease MRD in 50 consecutive MM patients who underwent an up-front tandem ASCT in our center, using a single-tube six-colors flow cytometry assay FC based on intra-cytoplasmic immunoglobulin cy-Ig light chains ratio evaluated on patient-specific plasma cells PC immune profile, in a real-life setting.

ResultsWith a sensitivity up to 10, clonal-PC were documented by FC in 36.4 % 12-33 of patients in conventional CR after second transplant. The number of flow MRD-negative patients significantly increased after induction and first ASCT, but not between first and second transplant. The 5-years progression-free survival 5ys-PFS of flow MRD-negative patients after second transplant was significantly better than patients who remained MRD-positive considering both all patients 5ys-PFS: 70 % vs 5 % and patients in CR according to standard criteria 5ys-PFS: 67 % vs 0 %.

ConclusionsFC remission through cy-Ig light ratio on PC sub-populations is a sensitive, highly informative, low-cost and routinely applicable MRD assay, a powerful tool in treatment response evaluation and a crucial marker of outcome in MM.

KeywordsMultiple myeloma Minimal residual disease Flow cytometry remission Light chains ratio Autologous stem cell transplant AbbreviationsASCTautologous stem cell transplant

BMbone marrow

CRcomplete remission.

cy-Igintra-cytoplasmatic immunoglobulin

FCflow cytometry

MMmultiple myeloma

MRDminimal residual disease

BDBBecton Dickinson Bioscience

PCplasma cells

PFSprogression-free survival

SAMsurface aberrant markers

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Autor: Iole Cordone - Francesco Marchesi - Serena Masi - Valentina Summa - Francesco Pisani - Roberta Merola - Giovanni Cigliana -

Fuente: https://link.springer.com/







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