Genome-wide identification and characterization of auxin response factor ARF family genes related to flower and fruit development in papaya Carica papaya L.Reportar como inadecuado

Genome-wide identification and characterization of auxin response factor ARF family genes related to flower and fruit development in papaya Carica papaya L. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genomics

, 16:901

First Online: 05 November 2015Received: 05 June 2015Accepted: 30 October 2015DOI: 10.1186-s12864-015-2182-0

Cite this article as: Liu, K., Yuan, C., Li, H. et al. BMC Genomics 2015 16: 901. doi:10.1186-s12864-015-2182-0


BackgroundAuxin and auxin signaling are involved in a series of developmental processes in plants. Auxin Response Factors ARFs is reported to modulate the expression of target genes by binding to auxin response elements AuxREs and influence the transcriptional activation of down-stream target genes. However, how ARF genes function in flower development and fruit ripening of papaya Carica papaya L. is largely unknown. In this study, a comprehensive characterization and expression profiling analysis of 11 C. papaya ARF CpARF genes was performed using the newly updated papaya reference genome data.

ResultsWe analyzed CpARF expression patterns at different developmental stages. CpARF1, CpARF2, CpARF4, CpARF5, and CpARF10 showed the highest expression at the initial stage of flower development, but decreased during the following developmental stages. CpARF6 expression increased during the developmental process and reached its peak level at the final stage of flower development. The expression of CpARF1 increased significantly during the fruit ripening stages. Many AuxREs were included in the promoters of two ethylene signaling genes CpETR1 and CpETR2 and three ethylene-synthesis-related genes CpACS1, CpACS2, and CpACO1, suggesting that CpARFs might be involved in fruit ripening via the regulation of ethylene signaling.

ConclusionsOur study provided comprehensive information on ARF family in papaya, including gene structures, chromosome locations, phylogenetic relationships, and expression patterns. The involvement of CpARF gene expression changes in flower and fruit development allowed us to understand the role of ARF-mediated auxin signaling in the maturation of reproductive organs in papaya.

KeywordsAuxin Auxin response factor Papaya Developmental process Fruit ripening AbbreviationsARFAuxin response factor

Aux-IAAAuxin-indole-3-acetic acid

AuxREAuxin response element

CTDC-terminal dimerization domain

DBDDNA binding domain

GH3Gretchen Hagen3

HMMHidden Markov model

kDak Dalton

SAURSmall auxin up RNA

MRMiddle region

MSMurashige and Skoog

ORFOpen reading frame

IAAIndole-3-acetic acid

TIBA2, 3, 5-triodobenzoic acid

qRT-PCRQuantitative real-time polymerase chain reaction

TBLASTBasic local alignment search tool

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-2182-0 contains supplementary material, which is available to authorized users.

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Autor: Kaidong Liu - Changchun Yuan - Haili Li - Wanhuang Lin - Yanjun Yang - Chenjia Shen - Xiaolin Zheng


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