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Journal of Experimental and Clinical Cancer Research

, 35:108

First Online: 04 July 2016Received: 29 February 2016Accepted: 23 June 2016DOI: 10.1186-s13046-016-0383-2

Cite this article as: Wu, D., Chen, C., Wu, Z. et al. J Exp Clin Cancer Res 2016 35: 108. doi:10.1186-s13046-016-0383-2

Abstract

BackgroundActivating transcription factor 2 ATF2 is a basic helix-loop-helix transcription factor, which has been shown to participate in the pathobiology of numerous cancers. However, the role of ATF2 in renal cell carcinoma RCC remains unclear.

MethodsATF2 knockdown and overexpression studies were performed in RCC cells to evaluate changes in cell viability, cell cycle, apoptosis, migration and invasion. Xenograft models were used to examine the tumorigenic and metastatic capability of RCC cells upon ATF2 suppression. The expression of ATF2 in human RCC samples was determined using immunohistochemistry on a tissue microarray.

ResultsATF2 knockdown in RCC cells reduced their proliferative and metastatic potentials, whereas ATF2 overexpression enhanced these properties. Mechanistic studies revealed that the transcription of CyclinB1, CyclinD1, Snail and Vimentin was directly regulated by ATF2 in RCC cells. Moreover, ATF2 was shown to be highly expressed in RCC tissues, especially in tumors with metastases. High expression of ATF2 correlated with aggressive clinico-pathological characteristics and predicted poor prognosis of RCC patients.

ConclusionsATF2 exerts an oncogenic role in RCC and could serve as an important prognostic biomarker.

KeywordsRenal cell carcinoma ATF2 Proliferation Metastasis Prognosis Electronic supplementary materialThe online version of this article doi:10.1186-s13046-016-0383-2 contains supplementary material, which is available to authorized users.

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Autor: Deng-shuang Wu - Cheng Chen - Zhen-jie Wu - Bing Liu - Li Gao - Qing Yang - Wei Chen - Jun-ming Chen - Yi Bao - Le Qu -

Fuente: https://link.springer.com/







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