Metabolic characteristics of dominant microbes and key rare species from an acidic hot spring in Taiwan revealed by metagenomicsReportar como inadecuado




Metabolic characteristics of dominant microbes and key rare species from an acidic hot spring in Taiwan revealed by metagenomics - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genomics

, 16:1029

Prokaryote microbial genomics

Abstract

BackgroundMicrobial diversity and community structures in acidic hot springs have been characterized by 16S rRNA gene-based diversity surveys. However, our understanding regarding the interactions among microbes, or between microbes and environmental factors, remains limited.

ResultsIn the present study, a metagenomic approach, followed by bioinformatics analyses, were used to predict interactions within the microbial ecosystem in Shi-Huang-Ping SHP, an acidic hot spring in northern Taiwan. Characterizing environmental parameters and potential metabolic pathways highlighted the importance of carbon assimilatory pathways. Four distinct carbon assimilatory pathways were identified in five dominant genera of bacteria. Of those dominant carbon fixers, Hydrogenobaculum bacteria outcompeted other carbon assimilators and dominated the SHP, presumably due to their ability to metabolize hydrogen and to withstand an anaerobic environment with fluctuating temperatures. Furthermore, most dominant microbes were capable of metabolizing inorganic sulfur-related compounds abundant in SHP. However, Acidithiobacillus ferrooxidans was the only species among key rare microbes with the capability to fix nitrogen, suggesting a key role in nitrogen cycling. In addition to potential metabolic interactions, based on the 16S rRNAs gene sequence of Nanoarchaeum-related and its potential host Ignicoccus-related archaea, as well as sequences of viruses and CRISPR arrays, we inferred that there were complex microbe-microbe interactions.

ConclusionsOur study provided evidence that there were numerous microbe-microbe and microbe-environment interactions within the microbial community in an acidic hot spring. We proposed that Hydrogenobaculum bacteria were the dominant microbial genus, as they were able to metabolize hydrogen, assimilate carbon and live in an anaerobic environment with fluctuating temperatures.

AbbreviationsCRISPRClustered regularly interspaced short palindromic repeats

DSSDirect shot-gun sequencing

SHPShi-Huang-Ping

Kuei-Han Lin, Ben-Yang Liao and Hao-Wei Chang contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-2230-9 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Kuei-Han Lin - Ben-Yang Liao - Hao-Wei Chang - Shiao-Wei Huang - Ting-Yan Chang - Cheng-Yu Yang - Yu-Bin Wang - Yu-Teh Kir

Fuente: https://link.springer.com/







Documentos relacionados