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BMC Genomics

, 17:32

Multicellular invertebrate genomics

Abstract

BackgroundA complete genome sequence and the advent of genome editing open up non-traditional model organisms to mechanistic genetic studies. The mosquito Aedes aegypti is an important vector of infectious diseases such as dengue, chikungunya, and yellow fever and has a large and complex genome, which has slowed annotation efforts. We used comprehensive transcriptomic analysis of adult gene expression to improve the genome annotation and to provide a detailed tissue-specific catalogue of neural gene expression at different adult behavioral states.

ResultsWe carried out deep RNA sequencing across all major peripheral male and female sensory tissues, the brain and female ovary. Furthermore, we examined gene expression across three important phases of the female reproductive cycle, a remarkable example of behavioral switching in which a female mosquito alternates between obtaining blood-meals from humans and laying eggs. Using genome-guided alignments and de novo transcriptome assembly, our re-annotation includes 572 new putative protein-coding genes and updates to 13.5 and 50.3 % of existing transcripts within coding sequences and untranslated regions, respectively. Using this updated annotation, we detail gene expression in each tissue, identifying large numbers of transcripts regulated by blood-feeding and sexually dimorphic transcripts that may provide clues to the biology of male- and female-specific behaviors, such as mating and blood-feeding, which are areas of intensive study for those interested in vector control.

ConclusionsThis neurotranscriptome forms a strong foundation for the study of genes in the mosquito nervous system and investigation of sensory-driven behaviors and their regulation. Furthermore, understanding the molecular genetic basis of mosquito chemosensory behavior has important implications for vector control.

KeywordsMosquito Aedes aegypti mRNA-sequencing De novo genome assembly Host-seeking behavior Neural genes Chemosensory receptors Ion channels G protein-coupled receptors Gonotrophic cycle Neurogenetics Abbreviations5-HT5-hydroxytryptamine serotonin

bpbase pair

CDScoding sequence

CO2carbon dioxide

CVcoefficient of variation

DAdopamine

Ddcdopamine decarboxylase

DEG-ENaCdegenerin-epithelial sodium channels

DNAdeoxyribonucleic acid

EHeclosion hormone

ESTexpressed sequence tag

ETHecdysis-triggering hormone

GABAɣ-aminobutryic acid

IACUCInstitutional Animal Care and Use Committee

IRionotropic receptor

IRBInstitutional Review Board

kbkilobase

mmeter

MA plotplot using an M log ratios and A mean average scale

Mbmegabase

mLmilliliter

NPYneuropeptide Y

OBPodorant binding protein

ORodorant receptor

ORFopen reading frame

PCAprincipal component analysis

PCRpolymerase chain reaction

PPKpickpocket

RNAribonucleic acid

RNA-seqmRNA-sequencing

secseconds

TbhTyramine β hydroxylase

Tdctyrosine decarboxylase

Thtyrosine hydroxylase

TPMtranscripts per million

TRPtransient receptor potential

μLmicroliter

UTRuntranslated region

VSTvariance stabilizing transformation

wtrwwater witch

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-015-2239-0 contains supplementary material, which is available to authorized users.

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Autor: Benjamin J. Matthews - Carolyn S. McBride - Matthew DeGennaro - Orion Despo - Leslie B. Vosshall

Fuente: https://link.springer.com/







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