Influence of platelet-activating factor receptor PAFR on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortusReportar como inadecuado




Influence of platelet-activating factor receptor PAFR on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 16:70

First Online: 21 April 2016Received: 02 July 2015Accepted: 07 April 2016DOI: 10.1186-s12866-016-0685-8

Cite this article as: Lee, J.J., Simborio, H.L., Reyes, A.W.B. et al. BMC Microbiol 2016 16: 70. doi:10.1186-s12866-016-0685-8

Abstract

BackgroundBrucella abortus is an intracellular pathogen which can infect and persist in host cells through multiple interactions. Above all, its interaction to host cell receptor is important to understand the pathogenic mechanisms of B. abortus. Accordingly, we demonstrated that platelet-activating factor receptor PAFR affects host cell response against B. abortus infection.

ResultsFirst of all, B. abortus infection to macrophage induces secretion of platelet-activating factor PAF, which is a PAFR agonist. The stimulation of PAFR by PAF remarkably increases B. abortus uptake into macrophages. It induces Janus kinase 2 JAK2 and p38α phosphorylation, indicating that PAFR-mediated activation of JAK2 signaling leads to enhanced uptake of B. abortus. Moreover, the dynamics of F-actin polymerization revealed that PAFR-mediated B. abortus uptake is related with the reorganization of F-actin and JAK2. Upon B. abortus phagocytosis, reduced PAFR in the membrane and subsequently increased levels of PAFR colocalization with endosomes were observed which indicate that B. abortus uptake into macrophages allowed PAFR trafficking to endosomes.

ConclusionsThis study demonstrated that PAFR has a compelling involvement in B. abortus uptake as a promoter of phagocytosis, which is associated with JAK2 activation. Thus, our findings establish a novel insight into a receptor-related phagocytic mechanism of B. abortus.

KeywordsB. abortus Platelet-activating factor receptor JAK2 Phagocytosis AbbreviationsPAFRplatelet-activating factor receptor

JAK2Janus kinase 2

GPCRG protein-coupled receptor superfamily

LPCAT2lyso-phosphatidylcholine acyltransferase 2

MOImultiplicities of infection

CMEclathrin-mediated endocytosis

TLRToll-like receptor: PAMPs, pathogen-associated molecular patterns

PRRspattern recognition receptors

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-016-0685-8 contains supplementary material, which is available to authorized users.

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Autor: Jin Ju Lee - Hannah Leah Simborio - Alisha Wehdnesday Bernardo Reyes - Huynh Tan Hop - Lauren Togonon Arayan - Hu Jang

Fuente: https://link.springer.com/







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