Local transmission and global dissemination of New Delhi Metallo-Beta-Lactamase NDM: a whole genome analysisReportar como inadecuado




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BMC Genomics

, 17:452

Prokaryote microbial genomics

Abstract

BackgroundNew Delhi metallo-β-lactamase blaNDM, a plasmid-borne carbapenemase gene associated with significant mortality and severely limited treatment options, is of global public health concern as it is found in extremely diverse Gram-negative bacterial strains. This study thus aims to genetically characterize local and global spread of blaNDM.

MethodsTo investigate local transmission patterns in the context of a single hospital, whole genome sequencing data of the first 11 blaNDM-positive bacteria isolated in a local hospital were analyzed to: 1 identify and compare blaNDM-positive plasmids; and 2 study the phylogenetic relationship of the bacteria chromosomes. The global analysis was conducted by analyzing 2749 complete plasmid sequences including 39 blaNDM-positive plasmids in the NCBI database, where: 1 the plasmids were clustered based on their gene composition similarity; 2 phylogenetic study was conducted for each blaNDM-positive plasmid cluster to infer the phylogenetic relationship within each cluster; 3 gene transposition events introducing blaNDM into different plasmid backbones were identified; and 4 clustering pattern was correlated with the plasmids’ incompatibility group and geographical distribution.

ResultsAnalysis of the first 11 blaNDM-positive isolates from a single hospital revealed very low blaNDM-positive plasmid diversity. Local transmission was characterized by clonal spread of a predominant plasmid with 2 sporadic instances of plasmid introduction. In contrast to the low diversity locally, global blaNDM spread involved marked plasmid diversity with no predominant bacterial clone. Thirty-nine 1.4 % out of the 2749 complete plasmid sequences were blaNDM-positive, and could be resolved into 7 clusters, which were associated with plasmid incompatibility group and geographical distribution. The blaNDM gene module was witnessed to mobilize between different plasmid backbones on at least 6 independent occasions.

ConclusionsOur analysis revealed the complex genetic pathways of blaNDM spread, with global dissemination characterized mainly by transposition of the blaNDM gene cassette into varied plasmids. Early local transmission following plasmid introduction is characterized by plasmid conjugation and bacterial spread. Our findings emphasize the importance of plasmid molecular epidemiology in understanding blaNDM spread.

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-016-2740-0 contains supplementary material, which is available to authorized users.

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Autor: Wei Xin Khong - Eryu Xia - Kalisvar Marimuthu - Wenting Xu - Yik-Ying Teo - Eng Lee Tan - Shiyong Neo - Prabha Unny Kri

Fuente: https://link.springer.com/







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