Serum HOTAIR as a novel diagnostic biomarker for esophageal squamous cell carcinomaReport as inadecuate

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Molecular Cancer

, 16:75

First Online: 04 April 2017Received: 02 November 2016Accepted: 27 March 2017DOI: 10.1186-s12943-017-0643-6

Cite this article as: Wang, W., He, X., Zheng, Z. et al. Mol Cancer 2017 16: 75. doi:10.1186-s12943-017-0643-6


BackgroundEarly diagnosis of esophageal squamous cell carcinoma ESCC is an important issue to improve the prognosis. HOX transcript antisense RNA HOTAIR, a long noncoding RNA lncRNA expressed from the HOXC locus, has been recently revealed as an oncogenic regulator in ESCC. This study aimed to investigate whether serum HOTAIR is involved in the diagnosis of ESCC.

MethodsIn this study, we detected serum HOTAIR expression in 50 patients with ESCC including 42 tumor resection and 8 without surgery and 20 healthy volunteers to investigate the role of serum HOTAIR in ESCC using the quantitative real-time polymerase chain reaction qRT-PCR method.

ResultsClinical data indicated that serum HOTAIR were correlated with TNM stage. The expression level of serum HOTAIR 0.189 ± 0.010 was significantly higher in ESCC patients compared with that of healthy controls 0.055 ± 0.008, P < 0.01. The ROC curve analysis yielded an area under the ROC curve AUC value of 0.793 95% CI: 0.692 to 0.895, P < 0.01. Also, the serum HOTAIR expression level decreased obviously in postoperative samples one month after the surgery compared to preoperative specimens. Moreover, there was a significant correlation between serum HOTAIR expression and the expression of HOTAIR in ESCC tissue according to Pearson correlation analysis.

ConclusionsOur study, for the first time, demonstrated that serum HOTAIR might serve as a potential biomarker for the diagnosis of ESCC.

KeywordsEsophageal squamous cell carcinoma Long noncoding RNA HOTAIR Biomarker Diagnosis  Download fulltext PDF

Author: Wenjian Wang - Xiaotian He - Zehua Zheng - Xiaofan Ma - Xueting Hu - Duoguang Wu - Minghui Wang


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