Tracking dendritic cell migration into lymph nodes by using a novel PET probe 18F-tetrafluoroborate for sodium-iodide symporterReportar como inadecuado

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EJNMMI Research

, 7:32

First Online: 04 April 2017Received: 02 February 2017Accepted: 22 March 2017DOI: 10.1186-s13550-017-0280-5

Cite this article as: Lee, S.B., Lee, H.W., Lee, H. et al. EJNMMI Res 2017 7: 32. doi:10.1186-s13550-017-0280-5


BackgroundRecently, F-tetrafluoroborate TFB was used as a substrate for the human sodium-iodide symporter hNIS reporter gene. This study evaluated the feasibility of performing molecular-genetic imaging by using the new radiotracer F-TFB for the hNIS gene, to track dendritic cell DC migration in live mice. A murine dendritic cell line DC2.4 co-expressing the hNIS and effluc genes DC-NF was established. To confirm the functional cellular expression of both effluc and NIS in the inoculated DC-NF cells by bio-medical imaging, combined bioluminescence imaging BLI and F-TFB positron emission tomography-computed tomography PET-CT imaging was performed after intramuscular injection with parental DCs and DC-NF cells. For DC-tracking, parental DCs or DC-NF cells were injected in the left or right mouse footpad, respectively, and F-TFB PET-CT and BLI were performed to monitor these cells in live mice.

ResultsIn vivo PET-CT and BLI showed a clear signal in DC-NF injection sites but not in parental DC injection sites. The signal intensity in DC-NF cells was correlated with time. In vivo F-TFB PET-CT imaging showed higher radiotracer activity in the draining popliteal lymph nodes DPLNs in DC-NF injection sites than those in DC injection sites on day 2. BLI also showed DC-NF cell migration to the DPLNs on day 2 after the injection.

ConclusionsMigration of DCs to the lymph nodes was successfully monitored using F-TFB PET-CT imaging of the NIS gene and optical imaging of the effluc gene in live mice. These data support the feasibility of using F-TFB as a substrate for hNIS reporter gene imaging to track the migration of DCs to the lymph nodes in live animals. The use of F-TFB may facilitate enhanced PET imaging of the hNIS reporter gene in small animals and humans in future studies.

KeywordsF-tetrafluoroborate Sodium-iodide symporter Dendritic cell Immunotherapy AbbreviationsAPCAllophycocyanin

BLIBioluminescence imaging

CCKCell counting kit

CTComputed tomography

DCsDendritic cells

DPLNsDraining popliteal lymph nodes

EGFPGreen fluorescent protein

HBSSHank’s balanced salt solution

hNISHuman sodium-iodide symporter

KClO4Potassium perchlorate

MHCMajor histocompatibility complex


PETPositron emission tomography

ROIRegion of interest

SPECTSingle-photon emission computed tomography


TLCThin-layer chromatography

TNFTumor necrosis factor

VOIsVolumes of interest

Electronic supplementary materialThe online version of this article doi:10.1186-s13550-017-0280-5 contains supplementary material, which is available to authorized users.

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Autor: Sang Bong Lee - Ho Won Lee - Hongje Lee - Yong Hyun Jeon - Sang-Woo Lee - Byeong-Cheol Ahn - Jaetae Lee - Shin Young J


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