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Exosomes, Bronchopulmonary Dysplasia, Stem cells, Endothelial Colony Forming Cells

Rajabali, Saima Nasiruddin

Supervisor and department: Dr. Bernard Thebaud, Department of Pediatrics

Examining committee member and department: Dr. Gary Lopaschuk, Department of Pediatrics Dr. John Greer, Department of Physiology Dr. Jason Dyck, Department of Pediatrics

Department: Medical Sciences-Paediatrics


Date accepted: 2013-09-26T11:13:09Z

Graduation date: 2013-11

Degree: Master of Science

Degree level: Master's

Abstract: Bronchopulmonary dysplasia BPD, a chronic lung disease of prematurity, results in alveolar simplification and respiratory distress in the newborn. Vascular component is implicated. Lung damage involves a deficiency in the number and function of progenitor cells. We hypothesize that endothelial colony forming cells ECFCs exist in the developing human lung, are impaired in hyperoxia and secrete exosomes. ECFCs from human fetal lung expressed CD31, CD105, CD144, CD146 and were negative for CD14 and CD45. In hyperoxic conditions, cord formation and clonogenic potential was impaired. Mesenchymal stem cell conditioned media MSC CdM improved clonogenic potential. Exosomes were isolated from human cord blood derived ECFC CdM and characterized using electron microscopy and protein expression.This study provides novel finding that ECFCs exist in human fetal lung and their function is impaired in hyperoxia. They may exert their effect by exosomes. This provides a rationale for use of exogenous stem cells in BPD.

Language: English

DOI: doi:10.7939-R30R9MB0S

Rights: Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.

Author: Rajabali, Saima Nasiruddin

Source: https://era.library.ualberta.ca/


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