Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern VietnamReportar como inadecuado

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BMC Microbiology

, 16:208

Applied microbiology


BackgroundEnteroaggregative EAEC and Shiga-toxin producing Escherichia coli STEC are a major cause of diarrhea worldwide. E. coli carrying both virulence factors characteristic for EAEC and STEC and producing extended-spectrum beta-lactamase caused severe and protracted disease during an outbreak of E. coli O104:H4 in Europe in 2011. We assessed the opportunities for E. coli carrying the aggR and stx genes to emerge in ‘backyard’ farms in south-east Asia.

ResultsFaecal samples collected from 204 chicken farms; 204 farmers and 306 age- and gender-matched individuals not exposed to poultry farming were plated on MacConkey agar plates with and without antimicrobials being supplemented. Sweep samples obtained from MacConkey agar plates without supplemented antimicrobials were screened by multiplex PCR for the detection of the stx1, stx2 and aggR genes. One chicken farm sample each 0.5 % contained the stx1 and the aggR gene. Eleven 2.4 % human faecal samples contained the stx1 gene, 2 samples 0.4 % contained stx2 gene, and 31 6.8 % contained the aggR gene. From 46 PCR-positive samples, 205 E. coli isolates were tested for the presence of stx1, stx2, aggR, wzxO104 and fliCH4 genes. None of the isolates simultaneously contained the four genetic markers associated with E. coli O104:H4 epidemic strain aggR, stx2, wzxO104 and fliCH4. Of 34 EAEC, 64.7 % were resistant to 3-generation cephalosporins.

ConclusionThese results indicate that in southern Vietnam, the human population is a more likely reservoir of aggR and stx gene carrying E. coli than the chicken population. However, conditions for transmission of isolates and-or genes between human and animal reservoirs resulting in the emergence of highly virulent E. coli strains are still favorable, given the nature of‘backyard’ farms in Vietnam.

KeywordsEAEC STEC E. coli Chicken Humans Vietnam AbbreviationsEAECEnteroaggregative Escherichia coli

EHECEnterohaemorrhagic Escherichia coli

PMCPreventive Medicine Centre of Tien Giang

SDAHSub-Department of Animal Health of Tien Giang

STECShiga toxin Stx-producing Escherichia coli

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-016-0827-z contains supplementary material, which is available to authorized users.

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Autor: Nguyen Vinh Trung - Hoang Ngoc Nhung - Juan J. Carrique-Mas - Ho Huynh Mai - Ha Thanh Tuyen - James Campbell - Nguyen 


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