Tissue localization of GM-CSF receptor in bovine ovarian follicles and its role on glucose uptake by mural granulosa cellsReportar como inadecuado

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The granulocyte-macrophage colony stimulating factor GM-CSF is a multifunctional cytokine implicated in proliferation, differentiation, and activation of several cell types including those involved in hematopoiesis and reproduction. In the present study, the expression of the alpha- and beta-subunit genes of GM-CSF receptor during follicular development in cattle was assessed. The spatial association of alpha- and beta-subunits of GM-CSF with follicle stimulating hormone receptor FSHR and 3 beta-hydroxysteroid dehydrogenase 3 beta-HSD, and the temporal associations with gene expression of hexose transporters GLUTs in granulosa cells of cattle were also evaluated. The effect of GM-CSF on the functionality of hexose transporters was also determined in an in vitro primary culture of granulosa cells. The spatial association of subunits of the GM-CSF receptor with 3 beta-HSD and FSHR suggests a potential steroidogenic regulation of GM-CSF in granulosa cells. Immunodetection of GLUTs and uptake kinetic assays confirmed expression and functionality of these genes for hexose transporters in granulosa cells of cattle. Treatment of granulosa cells with GM-CSF, FSH or insulin- like growth factor-I IGF-I alone increased 2-deoxyglucose DOG or 3-0-methylglucose OMG uptake; however, when cells were treated with various combination of these factors there were no additive effect. Unexpectedly, the combination of GM-CSF and FSH decreased DOG uptake compared to FSH treatment alone. Thus, the expression pattern of GM-CSF receptor subunit genes during follicle development in cattle and promotion of DOG and OMG uptake in granulosa cells indicate a role for GM-CSF, FSH and-or IGF-I alone in regulating granulosa cell metabolic activity, specifically by promoting glucose uptake.Indización

Artículo de publicación ISI

Autor: Peralta Troncoso, Óscar; - Bucher, Danai; - Angulo, C.; - Castro, M. A.; - Ratto, M. H.; - Concha, Il.; -

Fuente: http://repositorio.uchile.cl/


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