High fat diet induced atherosclerosis is accompanied with low colonic bacterial diversity and altered abundances that correlates with plaque size, plasma A-FABP and cholesterol: a pilot study of high fat diet and its intervention Reportar como inadecuado




High fat diet induced atherosclerosis is accompanied with low colonic bacterial diversity and altered abundances that correlates with plaque size, plasma A-FABP and cholesterol: a pilot study of high fat diet and its intervention - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 16:264

Applied microbiology

Abstract

BackgroundAtherosclerosis appears to have multifactorial causes – microbial component like lipopolysaccharides LPS and other pathogen associated molecular patterns may be plausible factors. The gut microbiota is an ample source of such stimulants, and its dependent metabolites and altered gut metagenome has been an established link to atherosclerosis. In this exploratory pilot study, we aimed to elucidate whether microbial intervention with probiotics L. rhamnosus GG LGG or pharmaceuticals telmisartan TLM could improve atherosclerosis in a gut microbiota associated manner.

MethodsAtherosclerotic phenotype was established by 12 weeks feeding of high fat HF diet as opposed to normal chow diet ND in apolipoprotein E knockout ApoE mice. LGG or TLM supplementation to HF diet was studied.

ResultsBoth LGG and TLM significantly reduced atherosclerotic plaque size and improved various biomarkers including endotoxin to different extents. Colonial microbiota analysis revealed that TLM restored HF diet induced increase in Firmicutes-Bacteroidetes ratio and decrease in alpha diversity; and led to a more distinct microbial clustering closer to ND in PCoA plot. Eubacteria, Anaeroplasma, Roseburia, Oscillospira and Dehalobacteria appeared to be protective against atherosclerosis and showed significant negative correlation with atherosclerotic plaque size and plasma adipocyte – fatty acid binding protein A-FABP and cholesterol.

ConclusionLGG and TLM improved atherosclerosis with TLM having a more distinct alteration in the colonic gut microbiota. Altered bacteria genera and reduced alpha diversity had significant correlations to atherosclerotic plaque size, plasma A-FABP and cholesterol. Future studies on such bacterial functional influence in lipid metabolism will be warranted.

KeywordsAtherosclerosis Probiotics LGG Telmisartan Gut microbitoa AbbreviationsA-FABPAdipocyte fatty acid binding protein

ApoE−-−Apolipoprotein E knockout

ƔBBƔ-butyrobetaine

GIGastrointestinal

HFHigh fat

LGGLactobacillus rhamnosus GG

LPSLipopolysaccharides

MMPsMetalloproteinases

NDNormal diet

OTUOperational taxonomic units

PAMPsPathogen associated molecular patterns

PCoAPrincipal coordinates

PPARƔPerixosome proliferator-activated receptor Ɣ

QIIMEQuantitative insights into microbial ecology

sE-selectinSoluble E-selectin

sICAM-1Soluble intercellular adhesion molecule – 1

sVCAM-1Soluble vascular cell adhesion molecule – 1

TLMTelmisartan

TMAOTrimethylamine-N-oxide

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Autor: Yee Kwan Chan - Manreetpal Singh Brar - Pirkka V. Kirjavainen - Yan Chen - Jiao Peng - Daxu Li - Frederick Chi-Ching Le

Fuente: https://link.springer.com/







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