The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional historiesReportar como inadecuado

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BMC Microbiology

, 16:266

Microbe-host interactions and microbial pathogenicity


BackgroundThe better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with Specific Growth Rate SGR and Hypoxia Resistance Time HRT in European sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challenge in a common garden approach at the juvenile stage.

ResultsA diet deficient in essential fatty acids LH lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient diet with high starch supply HG reduced SGR to a lesser extent than LH, but it did not affect HRT. In overall average, 94 % of pyrosequencing reads corresponded to Proteobacteria, and the differences in Operational Taxonomy Units OTUs composition were mildly significant between experimental groups, mainly due to high individual variability. The highest and the lowest Bray-Curtis indices of intra-group similarity were observed in the two groups fed standard starter diet, and then mixed before the final dietary challenge with fish already exposed to the nutritional deficiency at first feeding 0.60 and 0.42 with diets HG and LH, respectively. Most noticeably, the median percentage of Escherichia-Shigella OTU 1 was less in the group LH with standard starter diet. Disregarding the nutritional history of each individual, strong correlation appeared between 1 OTU richness and SGR, and 2 dominance index and HRT. The two physiological traits correlated also with the relative abundance of distinct OTUs positive correlations: Pseudomonas sp. OTU 3 and Herbaspirillum sp. OTU 10 with SGR, Paracoccus sp. OTU 4 and Vibrio sp. OTU 7 with HRT; negative correlation: Rhizobium sp. OTU 9 with HRT.

ConclusionsIn sea bass, gut microbiota characteristics and physiological traits of individuals are linked together, interfering with nutritional history, and resulting in high variability among individual microbiota. Many samples and tank replicates seem necessary to further investigate the effect of experimental treatments on gut microbiota composition, and to test the hypothesis whether microbiotypes may be delineated in fish.

KeywordsHost-microbe interaction 16S rRNA Pyrosequencing Autochthonous bacteria Alternative feed ingredients Physiological status AbbreviationsANOVAAnalysis Of VAriance

BlastBasic local alignment search tool

CControl diet C1 and C2, also used to name experimental groups

cDNAcomplementary DNA

DGGEDenaturing Gradient Gel Electrophoresis

DNADeoxyribo-Nucleic Acid

dphDay post hatch

FROGSFind Rapidly OTU with Galaxy Solution

HGHyper-Glucidic diet HG1 and HG2, also used to name experimental groups

HRTHypoxia Resistance Time

HUFAHighly poly-Unsaturated Fatty Acids

LDALinear Discriminant Analysis

LEfSeLinear discriminant Effective Size

LHLow-HUFA diet LH1 and LH2, also used to name experimental groups

OTUOperational Taxonomy Unit

PASTPAleontological STatistics software package

PCRPolymerase Chain Reaction

PERMANOVAPERmutational Multivariate Analysis Of Variance

PLSPartial Least Squares

RDPRibosomal Database Project

rRNAribosomal Ribo-Nucleic Acid

RT-PCRReverse Transcription Polymerase Chain Reaction

SEStandard error

SGRSpecific Growth Rate

sPLSsparse Partial Least Squares

Electronic supplementary materialThe online version of this article doi:10.1186-s12866-016-0885-2 contains supplementary material, which is available to authorized users.

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Autor: François-Joël Gatesoupe - Christine Huelvan - Nicolas Le Bayon - Hervé Le Delliou - Lauriane Madec - Olivier Mouchel - Pa


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