A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomicsReport as inadecuate

A shift in the virulence potential of Corynebacterium pseudotuberculosis biovar ovis after passage in a murine host demonstrated through comparative proteomics - Download this document for free, or read online. Document in PDF available to download.

BMC Microbiology

, 17:55

Microbial genetics, genomics and proteomics


BackgroundCorynebacterium pseudotuberculosis biovar ovis, a facultative intracellular pathogen, is the etiologic agent of caseous lymphadenitis in small ruminants. During the infection process, C. pseudotuberculosis changes its gene expression to resist different types of stresses and to evade the immune system of the host. However, factors contributing to the infectious process of this pathogen are still poorly documented. To better understand the C. pseudotuberculosis infection process and to identify potential factors which could be involved in its virulence, experimental infection was carried out in a murine model using the strain 1002 ovis and followed by a comparative proteomic analysis of the strain before and after passage.

ResultsThe experimental infection assays revealed that strain 1002 ovis exhibits low virulence potential. However, the strain recovered from the spleen of infected mice and used in a new infection challenge showed a dramatic change in its virulence potential. Label-free proteomic analysis of the culture supernatants of strain 1002 ovis before and after passage in mice revealed that 118 proteins were differentially expressed. The proteome exclusive to the recovered strain contained important virulence factors such as CP40 proteinase and phospholipase D exotoxin, the major virulence factor of C. pseudotuberculosis. Also, the proteome from recovered condition revealed different classes of proteins involved in detoxification processes, pathogenesis and export pathways, indicating the presence of distinct mechanisms that could contribute in the infectious process of this pathogen.

ConclusionsThis study shows that C. pseudotuberculosis modifies its proteomic profile in the laboratory versus infection conditions and adapts to the host context during the infection process. The screening proteomic performed us enable identify known virulence factors, as well as potential proteins that could be related to virulence this pathogen. These results enhance our understanding of the factors that might influence in the virulence of C. pseudotuberculosis.

KeywordsCorynebacterium pseudotuberculosis Bacterial label-free proteomic Caseous lymphadenitis Bacterial virulence Serial passage Extracellular proteins Electronic supplementary materialThe online version of this article doi:10.1186-s12866-017-0925-6 contains supplementary material, which is available to authorized users.

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Author: Wanderson M. Silva - Fernanda A. Dorella - Siomar C. Soares - Gustavo H. M. F. Souza - Thiago L. P. Castro - Núbia 

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