Detection of MUC1-Expressing Ovarian Cancer by C595 Monoclonal Antibody-Conjugated SPIONs Using MR ImagingReportar como inadecuado




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The Scientific World JournalVolume 2013 2013, Article ID 609151, 7 pages

Research Article

Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Department of Medical Physics and Radiation Technology, School of Paramedical Sciences, Bushehr University of Medical Sciences, Bushehr, Iran

Received 5 August 2013; Accepted 1 September 2013

Academic Editors: A. Ciarmiello and B. Schaller

Copyright © 2013 Daryoush Shahbazi-Gahrouei and Mohammad Abdolahi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study is to find out the development and application of MUC1-expressing ovarian cancer OVCAR3 by C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles SPIONs using MR imaging. At the end, its use as a nanosized contrast agent MR imaging probe for ovarian cancer detection was investigated. The strategy is to use SPIONs attached to C595 mAb that binds to the MUC1, to specifically detect ovarian cancer cells. Anticancer effects and MR imaging parameters of the prepared nanoconjugate was investigated both under in vitro and in vivo experiments. The characterization of nanoconjugate includes its size, cell toxicity, flow cytometry, Prussian blue staining test and its cellular uptake as well as its biodistribution, and MR imaging was also investigated. The findings of the study showed good tumor accumulation and detection, no in vivo toxicity, and potential selective antiovarian cancer activity. Overall, based on the findings SPIONs-C595 nanosized probe is a selective ovarian molecular imaging modality. Further subsequent clinical trials appear warranted.





Autor: Daryoush Shahbazi-Gahrouei and Mohammad Abdolahi

Fuente: https://www.hindawi.com/



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