A pilot study to determine the feasibility of collecting amniotic fluid samples from women during labour and measuring amniotic fluid lactate at point of careReportar como inadecuado




A pilot study to determine the feasibility of collecting amniotic fluid samples from women during labour and measuring amniotic fluid lactate at point of care - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Research Notes

, 6:112

First Online: 26 March 2013Received: 17 August 2012Accepted: 18 March 2013DOI: 10.1186-1756-0500-6-112

Cite this article as: Hall, B., Iwasenko, J., Moriatis, M. et al. BMC Res Notes 2013 6: 112. doi:10.1186-1756-0500-6-112

Abstract

BackgroundThe level of lactate in amniotic fluid may provide useful clinical information when assessing progress of a woman’s labour and if so, a rapid, reliable method to assess amniotic fluid lactate is required in order to be clinically relevant. However, measuring lactate levels in amniotic fluid, using portable, handheld lactate meters may be less accurate than reference laboratory instruments designed to measure lactate levels in aqueous solutions. Prior to conducting a large study, we assessed recruitment, consent and sampling procedures, and the accuracy of a handheld lactate meter to measure lactate in amniotic fluid. We compared amniotic fluid lactate results obtained using the hand held Lactate Pro Arkray to results obtained using reference laboratory methods ABX Pentra 400 Horiba.

ResultsWe recruited 35 nulliparous women during their antenatal hospital visits and tested amniotic fluid samples collected from 20 labouring women. The handheld Lactate Pro meter was found accurate from 9–20 mmol-L with a Passing and Bablok regression of y = 0.18 + 0.97x 95% CI 0.76–1.45. Amniotic fluid lactate results remained reliable in the presence of potential contaminants commonly encountered during labour; obstetric lubricant, blood and meconium.

ConclusionThe measurement of amniotic fluid lactate using the Lactate Pro meter was reliable compared to reference laboratory methods for measuring lactate levels in amniotic fluid. The pilot study enabled the refinement of information, recruitment, consenting and sampling procedures prior to commencing a large cohort study.

KeywordsDystocia Lactate Amniotic fluid Labour Point of care Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-6-112 contains supplementary material, which is available to authorized users.

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Autor: Beverley Hall - Jenna Iwasenko - Mary Moriatis - William D Rawlinson - Mark B Tracy - Sally K Tracy

Fuente: https://link.springer.com/







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