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BMC Research Notes

, 5:694

First Online: 24 December 2012Received: 30 August 2012Accepted: 18 December 2012DOI: 10.1186-1756-0500-5-694

Cite this article as: Gallegos, I.B., Pérez-Campos, E., Martinez, M. et al. BMC Res Notes 2012 5: 694. doi:10.1186-1756-0500-5-694

Abstract

BackgroundFibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors.

FindingsLectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts.

ConclusionsOur results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

KeywordsAntigen TF Galectin-3 Fibroadenoma Breast cancer Plant lectins AbbreviationsAEC3-amino-9-ethyl-carbazole

CDRCarbohydrate-recognition domain

FITCFluorescein isothiocyanate

GalGalactose

GalNAcN-acetylgalactosamine

PBSPhosphate buffered saline

SerSerine

ThrThreonine

TFThomsen-Friedenreich antigen

UABJOUniversidad Autónoma -Benito Juárez- de Oaxaca

UNAMUniversidad Autónoma de México

USAUnited Satates of America.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-5-694 contains supplementary material, which is available to authorized users.

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Autor: Itandehui Belem Gallegos - Eduardo Pérez-Campos - Margarito Martinez - Miguel Ángel Mayoral - Laura Pérez - Sergio Agui

Fuente: https://link.springer.com/







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