Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli LipopolysaccharideReportar como inadecuado




Preliminary Characterization of the Transcriptional Response of the Porcine Intestinal Cell Line IPEC-J2 to Enterotoxigenic Escherichia coli, Escherichia coli, and E. coli Lipopolysaccharide - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Comparative and Functional GenomicsVolume 2010 2010, Article ID 469583, 11 pages

Research ArticleDivision of Livestock Nutrition Quality, Department of Biosystems, K.U.Leuven, 3001 Heverlee, Belgium

Received 25 August 2010; Accepted 5 November 2010

Academic Editor: Paul  Denny

Copyright © 2010 Marisa M. Geens and Theo A. Niewold. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

IPEC-J2, a promising in vitro model system, is not well characterized especially on the transcriptional level, in contrast to human counterparts. The aim of this study was to characterize the gene expression in IPEC-J2 cells when coincubated with enterotoxigenic Escherichia coli ETEC, nonpathogenic E. coli, and E. coli endotoxin. Apical infection of polarized IPEC-J2 monolayers caused a time-dependent decrease in transepithelial electrical resistance TEER. Microarray analysis showed up-regulation of interleukins when IPEC-J2 were cocultured with E. coli strains this has so far never been measured in this cell line. Highest IL8 expression was found with the ETEC strain possessing the F4 fimbrium, suggesting IPEC-J2 cells to be F4 receptor positive, confirmed in a brush border membrane adhesion assay. It is concluded that the innate immune responses to pathogens and LPS makes the IPEC-J2 cell line a suitable model for research on intestinal host pathogen interaction.





Autor: Marisa M. Geens and Theo A. Niewold

Fuente: https://www.hindawi.com/



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