Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial jointsReportar como inadecuado




Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial joints - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Theoretical Biology and Medical Modelling

, 9:47

First Online: 20 November 2012Received: 30 August 2012Accepted: 30 August 2012DOI: 10.1186-1742-4682-9-47

Cite this article as: Guo, Y., Chen, J., Xiong, X. et al. Theor Biol Med Model 2012 9: 47. doi:10.1186-1742-4682-9-47

Abstract

BackgroundRheumatoid arthritis RA, a chronic autoimmune disease, affects sufferers in many different ways. Treatment of this chronic condition is particularly challenging. Traditional Chinese Medicine TCM provides alternatives. Bizhongxiao decoction BZX is a TCM complex, which has been used clinically for many years to treat RA. The purpose of this study is to compare the effects of BZX decoction and its dismantled formulae on IL-1 and TNF-1 levels in rats with RA, and to elucidate its mechanism of action.

MethodsNinety healthy normal female SD rats were randomly divided into six groups: normal control, model, BZX decoction, and the three dismantled formulae I: heat-clearing and detoxication, II: dissipating dampness, and III: blood circulation promotion. Apart from the normal control group, the rats in each group were injected subcutaneously with bovine type II collagen and complete Freund adjuvant to establish a collagen-induced arthritis model, so that inhibition of foot swelling in the rats by BZX decoction and its dismantled formulae could be observed. Immunohistochemistry was used to assess the levels of the inflammatory cytokines IL-1 and TNF in synovial joints at various time points.

ResultsTwenty-one days after the model was established, the levels of TNF and IL-1 were significantly higher in the model group, BZX decoction group and dismantled formula groups I, II and III than in the normal controls P < 0.05. The levels of these cytokines were significantly higher in the model group than the BZX decoction or the three dismantled formula groups P <0.01. At longer times, the TNF and IL-1 levels in model group rose gradually; those in the BZX decoction and dismantled formula groups were gradually reduced. The cytokine levels in the BZX decoction group were lower than in the three dismantled formula groups and continued to decline.

ConclusionsBZX decoction and the three dismantled formulae examined down-regulated the inflammatory factors IL-1 and TNF in collagen-induced arthritis rat models, but BZX exerted the strongest effect.

KeywordsBizhongxiao decoction Rheumatoid arthritis Interleukin-1 Tumor necrosis factor Electronic supplementary materialThe online version of this article doi:10.1186-1742-4682-9-47 contains supplementary material, which is available to authorized users.

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Autor: Ya-jing Guo - Jiang Chen - Xin-gui Xiong - Dan Wu - Hao Zhu - Qing-hua Liang

Fuente: https://link.springer.com/







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