Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer PatientsReportar como inadecuado




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Disease Markers - Volume 24 2008, Issue 3, Pages 141-150

II. Department of Medicine, Semmelweis University, 1088 Budapest, Hungary

Received 28 February 2008; Accepted 28 February 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims: CytokeratinCK based real-time RT-PCR assays QRT-PCR are now available for peripheral blood circulating tumor cell CTC evaluations in colorectal cancerCRC patients. Results are non-existent for the application of these techniques to the determination of progression and therapy response in Dukes stage D CRC patients.

Patients and methods: Each month 30 ml peripheral blood of 30 Dukes D patients 17 with progression were drawn. CEA, CA19-9, CA72-A and TPA-M determinations were made. CK20, thymidilate synthaseTS QRT-PCR was performed, as well. Buffy coat was used for immunmagnetic cancer cell isolation and CTC counting. Correlation between elevated CTC and macroscopic progression within 3 months was determined by Chi

test.

Results: Microscopic CTC single cell, doublet, cluster number were found in correlation with CK20 QRT-PCR results p <0.01. There was a significant increase in microscopic CTC number, CK20 and decrease in TS QRT-PCR levels p < 0.05 inthe peripheral blood of the non-responder as compared to responder patients. Elevation of the CTC was in significant correlationwith macroscopic progression of the disease in 3 months p < 0.01.

Conclusions: CTC number reflects the chemotherapeutic sensitivity of CRC patients. Elevation of circulating tumor cell number in peripheral blood is in correlation with macroscopic progression.





Autor: Bela Molnar, Lajos Floro, Ferenc Sipos, Bernadett Toth, Lydia Sreter, and Zsolt Tulassay

Fuente: https://www.hindawi.com/



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