Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory FactorReportar como inadecuado

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BioMed Research International - Volume 2014 2014, Article ID 941486, 10 pages -

Research Article

Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology NIGEB, Pajoohesh Boulevard, P.O. Box 14965-161, Tehran 1417863171, Iran

Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box 14115-154, Tehran, Iran

Received 25 February 2014; Accepted 16 June 2014; Published 23 July 2014

Academic Editor: Thomas Lufkin

Copyright © 2014 Masoumeh Fakhr Taha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The expression pattern of pluripotency markers in adipose tissue-derived stem cells ADSCs is a subject of controversy. Moreover, there is no data about the signaling molecules that regulate these markers in ADSCs. In the present study, we studied the roles of leukemia inhibitory factor LIF and miR-302 in this regard. Freshly isolated mouse ADSCs expressed hematopoietic, mesenchymal, and pluripotency markers. One day after plating, ADSCs expressed OCT4 and Sox2 proteins. After three passages, the expression of hematopoietic and pluripotency markers decreased, while the expression of mesenchymal stem cell markers exhibited a striking rise. Both supplementation of culture media with LIF and transfection of the ADSCs with miR-302 family upregulated the expression levels of OCT4, Nanog, and Sox2 mRNAs. These findings showed that mouse adipose tissue contains a population of cells with molecular resemblance to embryonic stem cells, and LIF and miR-302 family positively affect the expression of pluripotency markers.

Autor: Masoumeh Fakhr Taha, Arash Javeri, Sara Rohban, and Seyed Javad Mowla

Fuente: https://www.hindawi.com/


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