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BioMed Research International - Volume 2014 2014, Article ID 950516, 9 pages -

Research Article

Department of Kinesiology and Health, Miami University, Oxford, OH 45056, USA

Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA

Received 30 October 2013; Revised 23 December 2013; Accepted 14 January 2014; Published 25 February 2014

Academic Editor: J. Timothy Lightfoot

Copyright © 2014 Helaine M. Alessio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lifelong physical inactivity is associated with morbidity in adulthood, possibly influenced by changes in gene and protein expressions occurring earlier in life. mRNA Affymetrix gene array and proteomic 2D-DIGE MALDI-TOF-MS analyses were determined in cardiac tissue of young 3 months and old 16 months Sprague-Dawley rats housed with no access to physical activity SED versus an exercise wheel EX. Unfavorable phenotypes for body weight, dyslipidemia, and tumorogenesis appeared more often in adult SED versus EX. No differentially expressed genes DEGs occurred between groups at 3 or 16 months. Within groups, SED and EX shared 215 age-associated DEGs. In SED, ten unique DEGs occurred with age; three had cell adhesion functions fn1, lgals3, ncam2. In EX, five unique DEGs occurred with age; two involved hypothalamic, pituitary, and gonadal hormone axis nrob2, xpnpep2. Protein expression involved in binding, sugar metabolic processes, and vascular regulation declined with age in SED KNT1, ALBU, GPX1, PYGB, LDHB, G3P, PYGM, PGM1, ENOB. Protein expression increased with age in EX for ATP metabolic processes MYH6, MYH7, ATP5J, ATPA and vascular function KNT1, ALBU, GPX1. Differences in select gene and protein expressions within sedentary and active animals occurred with age and contributed to distinct health-related phenotypes in adulthood.

Autor: Helaine M. Alessio, Hayden Ansinelli, Caitlyn Threadgill, and Ann E. Hagerman



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