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Cellular Oncology

, Volume 34, Issue 5, pp 443–450

First Online: 15 May 2011Accepted: 01 May 2011DOI: 10.1007-s13402-011-0047-3

Cite this article as: Uleberg, KE., Munk, A.C., Skaland, I. et al. Cell Oncol. 2011 34: 443. doi:10.1007-s13402-011-0047-3

Abstract

BackgroundCervical intraepithelial neoplasia CIN, a frequently encountered disease caused by Human Papilloma Virus HPV is often diagnosed in formaldehyde-fixed paraffin embedded FFPE punch biopsies. Since it is known that this procedure strongly affects the water-soluble proteins contained in the cervical tissue we decided to investigate whether a water-soluble protein-saving biopsy processing method can be used to support the diagnosis of normal and CIN.

MethodsCervical punch biopsies from 55 women were incubated for 24 h at 4°C in RPMI1640 medium for protein analysis prior to usual FFPE processing and p16 and Ki67-supported histologic consensus diagnosis was assessed. The biopsy supernatants were subjected to surface-enhanced laser desorption-ionization time of flight mass spectrometry SELDI-TOF MS for identifying differentially expressed proteins. Binary logistic regression and classification and regression trees CART were used to develop a classification model.

ResultsThe age of the patients ranged from 26 to 40 years median 29.7. The consensus diagnoses were normal cervical tissue n = 10 and CIN2-3 n = 45. The mean protein concentration was 1.00 and 1.09 mg-ml in the normal and CIN2-3 group, respectively. The peak detection and clustering process resulted in 40 protein peaks. Many of these peaks differed between the two groups, but only three had independent discriminating power. The overall classification results were 88%.

ConclusionsWater-soluble proteins sampled from punch biopsies are promising to assist the diagnosis of normal and CIN2-3.

KeywordsCervical intraepithelial neoplasia CIN Proteomics SELDI-TOF Mass spectrometry Kai-Erik Uleberg and Ane Cecilie Munk contributed equally to this work

This study was financially supported by a grant from the Folke Hermansen Foundation in 2007

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