The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal Orbignya martianaReport as inadecuate




The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal Orbignya martiana - Download this document for free, or read online. Document in PDF available to download.

The Scientific World Journal - Volume 2016 2016, Article ID 2808241, 9 pages -

Research Article

Biodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, Brazil

Department of Chemistry, Federal University of Viçosa, Avenida PH Holfs, 36570-000 Viçosa, MG, Brazil

Department of Nutrition, Federal University of Espírito Santo, Avenida Fernando Ferrari, No. 514, Goiabeiras, 29075-010 Vitória, ES, Brazil

Received 6 February 2016; Accepted 20 April 2016

Academic Editor: Faik Nuzhet Oktar

Copyright © 2016 Márcia Regina Ribeiro Alves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity and yield % where  mg g

and 74.2%; 68.26 mg g

and 68.6%; and 36.18 mg g

, 37.4% of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.





Author: Márcia Regina Ribeiro Alves, Abraham Damian Giraldo Zuñiga, Rita de Cássia Superbi Sousa, and Carmelita Zacchi Scolforo

Source: https://www.hindawi.com/



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