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International Journal of Polymer ScienceVolume 2015 2015, Article ID 280784, 8 pages

Research Article

Department of Biotechnology, Bapuji Institute of Engineering and Technology, Davangere 577004, India

Department of Biotechnology, B.V.B. College of Engineering and Technology, Hubballi 580031, India

Received 31 July 2015; Accepted 4 November 2015

Academic Editor: Valéria C. Santos-Ebinuma

Copyright © 2015 B. E. Rangaswamy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified as Gluconacetobacter sp. RV28, Enterobacter sp. RV11, and Pseudomonas sp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources using Gluconacetobacter sp. RV28. The strain produced 4.7 g-L of cellulose at optimum growth conditions of temperature 30°C, pH 6.0, sucrose 2%, peptone 0.5%, and inoculum density 5%. Characterization of microbial cellulose was done by scanning electron microscopy SEM.

Author: B. E. Rangaswamy, K. P. Vanitha, and Basavaraj S. Hungund

Source: https://www.hindawi.com/


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