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Computational and Mathematical Methods in MedicineVolume 2012 2012, Article ID 790281, 11 pages

Research ArticleDepartment of Mathematics, Natural and Computer Sciences, Hochschule Mittweida, University of Applied Sciences, Technikumplatz 17, 09648 Mittweida, Germany

Received 24 November 2011; Accepted 4 January 2012

Academic Editor: Silvina Matysiak

Copyright © 2012 Florian Heinke and Dirk Labudde. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetes insipidus DI is a rare endocrine, inheritable disorder with low incidences in an estimated one per 25,000–30,000 live births. This disease is characterized by polyuria and compensatory polydypsia. The diverse underlying causes of DI can be central defects, in which no functional arginine vasopressin AVP is released from the pituitary or can be a result of defects in the kidney nephrogenic DI, NDI. NDI is a disorder in which patients are unable to concentrate their urine despite the presence of AVP. This antidiuretic hormone regulates the process of water reabsorption from the prourine that is formed in the kidney. It binds to its type-2 receptor V2R in the kidney induces a cAMP-driven cascade, which leads to the insertion of aquaporin-2 water channels into the apical membrane. Mutations in the genes of V2R and aquaporin-2 often lead to NDI. We investigated a structure model of V2R in its bound and unbound state regarding protein stability using a novel protein energy profile approach. Furthermore, these techniques were applied to the wild-type and selected mutations of aquaporin-2. We show that our results correspond well to experimental water ux analysis, which confirms the applicability of our theoretical approach to equivalent problems.





Autor: Florian Heinke and Dirk Labudde

Fuente: https://www.hindawi.com/



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