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Cellular and Molecular Life Sciences

, Volume 67, Issue 20, pp 3489–3497

First Online: 08 July 2010Received: 04 April 2010Revised: 12 June 2010Accepted: 15 June 2010DOI: 10.1007-s00018-010-0440-5

Cite this article as: Canaria, C.A. & Lansford, R. Cell. Mol. Life Sci. 2010 67: 3489. doi:10.1007-s00018-010-0440-5

Abstract

Developmental biology investigations have evolved from static studies of embryo anatomy and into dynamic studies of the genetic and cellular mechanisms responsible for shaping the embryo anatomy. With the advancement of fluorescent protein fusions, the ability to visualize and comprehend how thousands to millions of cells interact with one another to form tissues and organs in three dimensions xyz over time t is just beginning to be realized and exploited. In this review, we explore recent advances utilizing confocal and multi-photon time-lapse microscopy to capture gene expression, cell behavior, and embryo development. From choosing the appropriate fluorophore, to labeling strategy, to experimental set-up, and data pipeline handling, this review covers the various aspects related to acquiring and analyzing multi-dimensional data sets. These innovative techniques in multi-dimensional imaging and analysis can be applied across a number of fields in time and space including protein dynamics to cell biology to morphogenesis.

KeywordsConfocal Two-photon Microscopy Time-lapse imaging Embryogenesis  Download fulltext PDF



Autor: Christie A. Canaria - Rusty Lansford

Fuente: https://link.springer.com/







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